Document Detail


Functional homology of gHs and gLs from EBV-related gamma-herpesviruses for EBV-induced membrane fusion.
MedLine Citation:
PMID:  17477951     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epstein-Barr virus (EBV) is a human gamma-herpesvirus that primarily infects B lymphocytes and epithelial cells. Entry of EBV into B cells requires the viral glycoproteins gp42, gH/gL and gB, while gp42 is not necessary for infection of epithelial cells. In EBV, gH and gL form two distinct complexes, a bipartite complex that contains only gH and gL, used for infection of epithelial cells, and a tripartite complex that additionally includes gp42, used for infection of B cells. The gH/gL complex is conserved within the herpesvirus family, but its exact role in entry and mechanism of fusion is not yet known. To understand more about the functionality of EBVgH/gL, we investigated the functional homology of gHs and gLs from human herpesvirus 8 (HHV8) and two primate (rhesus and marmoset) gamma-herpesviruses in EBV-mediated virus-free cell fusion assay. Overall, gHs and gLs from the more homologous primate herpesviruses were better at complementing EBV gH and gL in fusion than HHV8 gH and gL. Interestingly, marmoset gH was able to complement fusion with epithelial cells, but not B cells. Further investigation of this led to the discovery that EBVgH is the binding partner of gp42 in the tripartite complex and the absence of fusion with B cells in the presence of marmoset gH/gL is due to its inability to bind gp42.
Authors:
Jasmina Omerović; Richard Longnecker
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-05-02
Journal Detail:
Title:  Virology     Volume:  365     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-06-19     Completed Date:  2007-09-26     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  157-65     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, The Feinberg School of Medicine, Northwestern University, Ward 6-231, 303 E. Chicago Ave., Chicago, IL 60611, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / virology
CHO Cells
Cricetinae
Cricetulus
Epithelial Cells / cytology,  virology
Glycoproteins / chemistry*,  genetics,  metabolism*
Herpesvirus 4, Human / physiology*
Herpesvirus 8, Human / chemistry,  classification,  physiology*
Membrane Fusion / physiology*
Viral Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
CA117794/CA/NCI NIH HHS; CA62234/CA/NCI NIH HHS; CA73507/CA/NCI NIH HHS; CA93444/CA/NCI NIH HHS; R01 AI067048-03/AI/NIAID NIH HHS; R01 AI076183-07/AI/NIAID NIH HHS; R01 CA117794-01A1/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Viral Proteins
Comments/Corrections

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