Document Detail


Functional and histological changes in rat femoral arteries by HIFU exposure.
MedLine Citation:
PMID:  14597344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was an investigation of arterial contractility in response to high-intensity focused ultrasound (HIFU) and of histologic changes to the artery with various intensities of HIFU. We constructed a prototype HIFU transducer in combination with an imaging probe that provides color Doppler imaging and Doppler velocimetry. HIFU was applied through the skin to deep femoral arteries in left thighs of Sprague-Dawley rats; color images of the blood flow were used to aim the HIFU beam. Peak intensities used were 530, 1080, 2750 and 4300 W/cm2. The duration of each HIFU exposure was 5 s. HIFU was applied to five focal spots of each leg. These focal spots were aligned with a spacing of 1.0 mm so as to form a line across the artery. Blood flow occlusion was accomplished by HIFU at an intensity of 4300 W/cm2, but the flow continued with the lower intensities. Peak systolic velocities (PSVs) of blood flow as measured by Doppler velocimetry increased in the arteries to which HIFU had been applied at 1080 and 2750 W/cm2. The increase corresponded with HIFU intensity. Exposure to HIFU at 530 W/cm2 did not change the blood flow velocity. Histologic studies have demonstrated that exposure to HIFU at 2750 and 4300 W/cm2 leads to vacuolar degeneration and destruction of elastic fibers of the tunica media of the artery. Exposure at 1080 W/cm2 led to increased PSV, but did not induce histologic changes in the vessel wall. In conclusion, the response of the artery to HIFU varied with intensity. Vascular contraction without tissue degeneration occurred at low intensity; with increasing intensity, the tissue degeneration detectable in histology reduced the vascular diameter and, finally, at high intensity, the blood flow was occluded. Although these phenomena appeared to be mainly due to thermal effects, mechanical effects might have some role, particularly on vascular contraction.
Authors:
Tetsuya Ishikawa; Takashi Okai; Kazuaki Sasaki; Shin-ichiro Umemura; Rei Fujiwara; Miki Kushima; Mitsuyoshi Ichihara; Kiyotake Ichizuka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  29     ISSN:  0301-5629     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-11-04     Completed Date:  2004-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1471-7     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Showa University School of Medicine, Shinagawa, Tokyo, Japan. ishitetuishitetu@yahoo.co.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Flow Velocity
Female
Femoral Artery / pathology,  physiopathology*,  ultrasonography
Rats
Rats, Sprague-Dawley
Regional Blood Flow
Transducers
Tunica Media / pathology
Ultrasonic Therapy / instrumentation,  methods*
Ultrasonography, Doppler, Color
Vasoconstriction

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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