Document Detail


Functional heterogeneity of colonic adenocarcinoma mucins for inhibition of Entamoeba histolytica adherence to target cells.
MedLine Citation:
PMID:  9561779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mucins secreted from the gastrointestinal epithelium from the basis of the adherent mucus layer which is the host's first line of defense against invasion by Entamoeba histolytica. Galactose and N-acetyl-D-galactosamine residues of mucins specifically inhibit binding of the amebic 170 kDa heavy subunit Gal-lectin to target cells, an absolute prerequisite for pathogenesis. Herein we characterized the secretory mucins isolated from the human colon and from three human colonic adenocarcinoma cell lines: two with goblet cell-like (LS174T and T84) and one with absorptive cell-like morphology (Caco-2). By Northern blot analysis the intestinal mucin genes MUC2 and MUC3 were constitutively expressed by confluent LS174T and Caco-2 cells, whereas T84 cells only transcribed MUC2 and not MUC3 mRNA. 3H-glucosamine and 3H-threonine metabolically labeled proteins separated as high M, mucins in the void (Vo > 10(6) Da) of Sepharose-4B column chromatography and remained in the stacking gel of SDS-PAGE as depicted by fluorography. All mucin preparations contained high amounts of N-acetyl-glucosamine, galactose, N-acetyl-galactosamine, fucose and sialic acid, saccharides typical of the O-linked carbohydrate side chains. Mucin samples from the human colon and from LS174T and Caco-2 cells inhibited E. histolytica adherence to chinese hamster ovary cells, whereas mucins from T84 cells did not. These results suggest that genetic heterogeneity and/or posttranslational modification in glycosylation of colonic mucins can affect specific epithelial barrier function against intestinal pathogens.
Authors:
M U Göttke; K Keller; A Belley; R M Garcia; M A Hollingsworth; D R Mack; K Chadee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  45     ISSN:  1066-5234     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:    1998 Mar-Apr
Date Detail:
Created Date:  1998-06-04     Completed Date:  1998-06-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17S-23S     Citation Subset:  IM    
Affiliation:
Institute of Parasitology, McGill University, Québec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma, Mucinous / chemistry*,  physiopathology
Amino Acids / analysis
Animals
Blotting, Northern
CHO Cells
Caco-2 Cells
Cell Adhesion Molecules / chemistry*
Chromatography, Agarose
Chromatography, Gas
Colon / chemistry*
Colonic Neoplasms / chemistry*
Cricetinae
Electrophoresis, Polyacrylamide Gel
Entamoeba histolytica / chemistry*
Entamoebiasis / parasitology
Fluorometry
Gene Expression Regulation, Neoplastic
Host-Parasite Interactions / physiology
Humans
Mucins / chemistry*,  genetics,  physiology
RNA, Messenger / biosynthesis
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
DK 02205/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cell Adhesion Molecules; 0/Mucins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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