Document Detail


Functional hepatic recovery after xenotransplantation of cryopreserved fetal liver cells or soluble cell-factor administration in a cirrhotic rat model: are viable cells necessary?
MedLine Citation:
PMID:  17725601     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIM: Chronic liver failure results in the decrease of the number of functioning hepatocytes. It dictates the necessity of using exogenous viable cells or/and agents that can stimulate hepatic regenerative processes. Fetal liver contains both hepatic and hematopoietic stem cells with high proliferative potential, which may replace damaged cells. Also, immature cells produce fetal-specific factors which may support the injured liver. Our aim was to test the ability of human fetal liver cells and cell-free fetal-specific factors of non-hepatic origin to stimulate recovery processes in an experimental model of carbon tetrachloride-induced cirrhosis in rats. METHODS: Cirrhotic rats were intrasplenically injected with fetal liver cells (1 x 10(7) cells/0.3 mL medium) or cell-free fetal-specific factors (0.3 mL/1 mg protein). Control groups received medium alone. Serum indexes, hepatic functions, and morphology were evaluated for 15 days. RESULT: Human fetal liver cell transplantation was shown to abrogate the mortality of cirrhotic animals, to improve serum markers, and to restore liver mitochondrial function and detoxification. Morphological patterns of liver recovery were observed by histology. In comparison, an injection of fetal-specific factors produced similar functional recovery, whilst a more limited liver regeneration was observed by histology. CONCLUSIONS: The positive effects of fetal liver cell and cell-free fetal-specific factors in experimental cirrhosis may result from the presence of stage-specific factors activating hepatocellular repair.
Authors:
Olga V Ochenashko; Yurii V Nikitchenko; Nataliya A Volkova; Svetlana P Mazur; Alexander Y Somov; Barry J Fuller; Alexander Y Petrenko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-27
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  23     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-08-14     Completed Date:  2008-10-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  e275-82     Citation Subset:  IM    
Affiliation:
Department of Cryobiochemistry, Institute for Problems of Cryobiology and Cryomedicine, Kharkov National University, Kharkov, Ukraine. ochenol@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Bilirubin / blood
Carbon Tetrachloride
Cell Extracts / pharmacology*
Cell Respiration / drug effects
Cell Survival
Cryopreservation*
Cytochrome P-450 Enzyme System / metabolism
Embryonic Stem Cells* / drug effects,  enzymology,  pathology,  transplantation
Humans
Liver* / drug effects,  embryology,  enzymology,  pathology,  surgery
Liver Cirrhosis, Experimental* / chemically induced,  drug therapy,  pathology,  surgery
Liver Regeneration / drug effects*
Male
Mitochondria, Liver / drug effects
Rats
Serum Albumin / metabolism
Stem Cell Transplantation*
Thiobarbituric Acid Reactive Substances / metabolism
Time Factors
Transplantation, Heterologous
Grant Support
ID/Acronym/Agency:
069472//Wellcome Trust
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Serum Albumin; 0/Thiobarbituric Acid Reactive Substances; 56-23-5/Carbon Tetrachloride; 635-65-4/Bilirubin; 9035-51-2/Cytochrome P-450 Enzyme System

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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