| Functional hepatic recovery after xenotransplantation of cryopreserved fetal liver cells or soluble cell-factor administration in a cirrhotic rat model: are viable cells necessary? | |
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MedLine Citation:
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PMID: 17725601 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND AIM: Chronic liver failure results in the decrease of the number of functioning hepatocytes. It dictates the necessity of using exogenous viable cells or/and agents that can stimulate hepatic regenerative processes. Fetal liver contains both hepatic and hematopoietic stem cells with high proliferative potential, which may replace damaged cells. Also, immature cells produce fetal-specific factors which may support the injured liver. Our aim was to test the ability of human fetal liver cells and cell-free fetal-specific factors of non-hepatic origin to stimulate recovery processes in an experimental model of carbon tetrachloride-induced cirrhosis in rats. METHODS: Cirrhotic rats were intrasplenically injected with fetal liver cells (1 x 10(7) cells/0.3 mL medium) or cell-free fetal-specific factors (0.3 mL/1 mg protein). Control groups received medium alone. Serum indexes, hepatic functions, and morphology were evaluated for 15 days. RESULT: Human fetal liver cell transplantation was shown to abrogate the mortality of cirrhotic animals, to improve serum markers, and to restore liver mitochondrial function and detoxification. Morphological patterns of liver recovery were observed by histology. In comparison, an injection of fetal-specific factors produced similar functional recovery, whilst a more limited liver regeneration was observed by histology. CONCLUSIONS: The positive effects of fetal liver cell and cell-free fetal-specific factors in experimental cirrhosis may result from the presence of stage-specific factors activating hepatocellular repair. |
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Authors:
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Olga V Ochenashko; Yurii V Nikitchenko; Nataliya A Volkova; Svetlana P Mazur; Alexander Y Somov; Barry J Fuller; Alexander Y Petrenko |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-27 |
Journal Detail:
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Title: Journal of gastroenterology and hepatology Volume: 23 ISSN: 1440-1746 ISO Abbreviation: J. Gastroenterol. Hepatol. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-08-14 Completed Date: 2008-10-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: Australia |
Other Details:
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Languages: eng Pagination: e275-82 Citation Subset: IM |
Affiliation:
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Department of Cryobiochemistry, Institute for Problems of Cryobiology and Cryomedicine, Kharkov National University, Kharkov, Ukraine. ochenol@yahoo.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bilirubin / blood Carbon Tetrachloride Cell Extracts / pharmacology* Cell Respiration / drug effects Cell Survival Cryopreservation* Cytochrome P-450 Enzyme System / metabolism Embryonic Stem Cells* / drug effects, enzymology, pathology, transplantation Humans Liver* / drug effects, embryology, enzymology, pathology, surgery Liver Cirrhosis, Experimental* / chemically induced, drug therapy, pathology, surgery Liver Regeneration / drug effects* Male Mitochondria, Liver / drug effects Rats Serum Albumin / metabolism Stem Cell Transplantation* Thiobarbituric Acid Reactive Substances / metabolism Time Factors Transplantation, Heterologous |
| Grant Support | |
ID/Acronym/Agency:
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069472//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Serum Albumin; 0/Thiobarbituric Acid Reactive Substances; 56-23-5/Carbon Tetrachloride; 635-65-4/Bilirubin; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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