Document Detail

Functional expression of a proliferation-related ligand in hepatocellular carcinoma and its implications for neovascularization.
MedLine Citation:
PMID:  16094704     Owner:  NLM     Status:  MEDLINE    
AIM: To detect the expression of a proliferation-related ligand on human hepatocellular carcinoma (HCC) cell lines (SK-Hep1, HLE and HepG2) and in culture medium. METHODS: APRIL expression was analyzed by Western blotting in HCC cell lines. Effects of APRIL to cell count and angiogenesis were analyzed, too. RESULTS: Recombinant human APRIL (rhAPRIL) increased cell viability of HepG2 cells and, in HUVEC, rhAPRIL provided slight tolerance to cell death from serum starvation. Soluble APRIL (sAPRIL) from HLE cells increased after serum starvation, but did not change in SK-Hep1 or HepG2 cells. These cells showed down-regulation of VEGF after incubation with anti-APRIL antibody. Furthermore, culture medium from the HCC cells treated with anti-APRIL antibody treatment inhibited tube formation of HUVECs. CONCLUSION: Functional expression of APRIL might contribute to neovascularization via an upregulation of VEGF in HCC.
Hiroshi Okano; Katsuya Shiraki; Yutaka Yamanaka; Hidekazu Inoue; Tomoyuki Kawakita; Yukiko Saitou; Yumi Yamaguchi; Naoyuki Enokimura; Keiichi Ito; Norihiko Yamamoto; Kazushi Sugimoto; Kazumoto Murata; Takeshi Nakano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  11     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-11     Completed Date:  2005-11-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4650-4     Citation Subset:  IM    
First Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie 514-8507, Japan.
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MeSH Terms
Carcinoma, Hepatocellular / blood supply,  metabolism*
Cell Line, Tumor
Cell Proliferation / drug effects
Endothelium, Vascular / cytology,  drug effects,  metabolism
Liver Neoplasms / blood supply,  metabolism*
Membrane Proteins / metabolism*
Neovascularization, Pathologic
Recombinant Proteins / pharmacology
Tumor Necrosis Factor Ligand Superfamily Member 13
Tumor Necrosis Factor-alpha / metabolism*
Vascular Endothelial Growth Factor A / metabolism
Reg. No./Substance:
0/Ligands; 0/Membrane Proteins; 0/Recombinant Proteins; 0/TNFSF13 protein, human; 0/Tumor Necrosis Factor Ligand Superfamily Member 13; 0/Tumor Necrosis Factor-alpha; 0/Vascular Endothelial Growth Factor A

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