Document Detail


Functional expression of mouse insulin-like growth factor-I with food-grade vector in Lactococcus lactis NZ9000.
MedLine Citation:
PMID:  22332956     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  To functionally express the recombinant mouse insulin-like growth factor-I (rtmIGF-I) in Lactococcus lactis NZ9000 with a food-grade vector. Methods and Results:  The rtmIGF-I encoding sequence was inserted into secreted food-grade vector pLEB688 and transformed into Lc. lactis NZ9000. The expression of the recombinant protein rtmIGF-I was confirmed by tricine-SDS-PAGE analysis and Western blot. The concentration of this recombinant protein was 3 mg l(-1) in the medium fraction. Further experiment demonstrated that the recombinant protein was biologically active and promoted NIH3T3 cell proliferation in a concentration- dependent manner. Conclusions:  The rtmIGF-I was expressed in Lc. lactis and located into the medium fraction. The optimal final concentration which could promote NIH3T3 cell proliferation after incubation was 100 ng ml(-1) . Significance and Impact of the Study:  The rtmIGF-I was functionally expressed in Lc. lactis NZ9000 with a food-grade vector. Thus, the recombinant Lc. lactis NZ9000 could act as a host for the production of rtmIGF-I for further study. The recombinant strain could serve as an IGF-I delivery system. © 2012 The Authors Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
G Gao; J-J Qiao; C-H Yang; D-Z Jiang; R-Q Li; J-J Su; H-J Xu; X-M Zhang; Y-L Bai; M-Q Qiao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-14
Journal Detail:
Title:  Letters in applied microbiology     Volume:  -     ISSN:  1472-765X     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 The Society for Applied Microbiology.
Affiliation:
The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China.
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