| Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 8685264 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9, delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry. |
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Authors:
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P S Covello; D W Reed |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 111 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-08-20 Completed Date: 1996-08-20 Revised Date: 2010-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 223-6 Citation Subset: IM |
Affiliation:
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National Research Council, Plant Biotechnology Institute, Saskatoon, Saskatchewan, Canada. pcovello@pbi.nrc.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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enzymology,
genetics* Base Sequence Cloning, Molecular DNA Primers Fatty Acid Desaturases / genetics*, metabolism Molecular Sequence Data Oxidoreductases Acting on CH-CH Group Donors Saccharomyces cerevisiae / genetics Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.3.-/Oxidoreductases Acting on CH-CH Group Donors; EC 1.3.1.35/Phosphatidylcholine desaturase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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