Document Detail

Functional expression of connexin30 and connexin31 in the polarized human airway epithelium.
MedLine Citation:
PMID:  17428265     Owner:  NLM     Status:  MEDLINE    
Gap junctions are documented in the human airway epithelium but the functional expression and molecular identity of their protein constituents (connexins, Cx) in the polarized epithelium is not known. To address this question, we documented the expression of a family of epithelial Cx (Cx26, Cx30, Cx30.3, Cx31, Cx31.1, Cx32, Cx37, Cx40, and Cx43) in primary human airway epithelial cells (AEC) grown on porous supports. Under submerged conditions, AEC formed a monolayer of airway cells whereas the air-liquid interface induced within 30-60 days AEC differentiation into a polarized epithelium for up to 6-9 months. Maturation of AEC was associated with the down-regulation of Cx26 and Cx43. The well-differentiated airway epithelium exhibited gap junctional communication between ciliated and between ciliated and basal cells. Interestingly, Cx30 was mostly present between ciliated cells whereas Cx31 was found between basal cells. These results are supportive of the establishment of signal-selective gap junctions with maturation of AEC, likely contributing to support airway epithelium function. These results lay the ground for studying the role of Cx-mediated cell-cell communication during repair following AEC injury and exploring Cx-targeted interventions to modulate the healing process.
Ludovic Wiszniewski; Javier Sanz; Isabelle Scerri; Elena Gasparotto; Tecla Dudez; Jean Silvain Lacroix; Susanne Suter; Sabina Gallati; Marc Chanson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-11
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  75     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-28     Completed Date:  2007-07-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  382-92     Citation Subset:  IM    
Laboratory of Clinical Investigation III, Department of Pediatrics, Geneva University Hospitals, Geneva, Switzerland.
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MeSH Terms
Cell Communication
Cell Differentiation
Cells, Cultured
Connexins / genetics*
Gap Junctions / genetics,  metabolism
Gene Expression Regulation / physiology*
Mice, Inbred C57BL
Respiratory Mucosa / metabolism*,  ultrastructure
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Connexins; 0/GJB6 protein, human; 136362-16-8/GJB3 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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