Document Detail


Functional expression of NOD2 in freshly isolated human peripheral blood γδ T-cells.
MedLine Citation:
PMID:  21410503     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
γδ T-cells play an important role in anti-infective immunity. The major subset of human γδ T-cells selectively recognizes phosphorylated bacterial metabolites of the isoprenoid biosynthesis pathway, so-called phosphoantigens. The activation of γδ T-cells is modulated by functionally expressed innate immune receptors, notably Toll-like receptor 2 and 3. It was also reported that in vitro expanded γδ T-cells respond to muramyl dipeptide (MDP), the minimal peptidoglycan motif activating the nucleotide-binding oligomerization domain containing 2 (NOD2) receptor, although it is unknown whether ex vivo isolated human γδ T-cells express functional NOD2. Here we report that freshly isolated, highly purified peripheral blood γδ T-cells express NOD2 mRNA and detectable amounts of NOD2 protein. The biologically active MDP L-D isomer but not the inactive D-D isomer augmented the interferon-γ (IFN-γ) secretion in phosphoantigen-stimulated peripheral blood mononuclear cells. Moreover, a moderate but reproducible and statistically significant increase in IFN-γ secretion was also observed when highly purified peripheral blood γδ T-cells were activated by T-cell receptor cross-linking in the presence of MDP. Taken together, our results indicate that in addition to the T-cell receptor and Toll-like receptors, circulating human γδ T-cells express NOD2 as a third class of pattern recognition receptor for sensing bacterial products.
Authors:
Lothar Marischen; Daniela Wesch; Hans-Heinrich Oberg; Philip Rosenstiel; Ahmad Trad; Mohammad Shomali; Joachim Grötzinger; Ottmar Janssen; Vladimir Tchikov; Stefan Schütze; Dieter Kabelitz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-15
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  -     ISSN:  1365-3083     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Blackwell Publishing Ltd.
Affiliation:
Institute of Immunology Institute of Clinical Molecular Biology Institute of Biochemistry, University of Kiel, 24105 Kiel, Germany.
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