| Functional exchange of an oncoretrovirus and a lentivirus matrix protein. | |
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MedLine Citation:
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PMID: 8207817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To map functional domains in the retroviral Gag protein we have constructed chimeric viruses where regions of the murine leukemia virus (MuLV) Gag protein have been replaced with analogous sequences from human immunodeficiency virus type 1 (HIV-1). Here we describe the chimeric virus MuLV(MAHIV) which contains the HIV-1 matrix (MA) protein in place of the MuLV MA. MuLV(MAHIV) is infectious but grows at a reduced rate compared with wild-type MuLV. We found that the partial defect in replication of the chimeric virus is at a late stage in the viral life cycle. The MuLV(MAHIV) Gag proteins are distributed aberrantly within cells and are not associated with cellular membranes. Unlike MuLV, HIV-1 is able to integrate into growth-arrested cells. Incorporation of the HIV-1 MA, which is known to play a role in infection of nondividing cells, does not enable MuLV(MAHIV) to be expressed in growth-arrested cells. While it possesses no amino acid homology, we found that the HIV-1 MA can efficiently replace the MuLV matrix protein in infection. |
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Authors:
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C A Deminie; M Emerman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 68 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-07-13 Completed Date: 1994-07-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 4442-9 Citation Subset: IM; X |
Affiliation:
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Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Cell Division Cell Line Chimera Gene Products, gag / genetics, metabolism, physiology* HIV Antigens / genetics, physiology* HIV-1 / genetics, metabolism, physiology* Half-Life Hela Cells Humans Kinetics Leukemia Virus, Murine / genetics, physiology* Mice Protein Precursors / metabolism Tumor Cells, Cultured Viral Matrix Proteins / genetics, physiology* Viral Proteins* Virion Virus Replication gag Gene Products, Human Immunodeficiency Virus |
| Grant Support | |
ID/Acronym/Agency:
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AI08587/AI/NIAID NIH HHS; R01 AI30927/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gene Products, gag; 0/HIV Antigens; 0/Protein Precursors; 0/Viral Matrix Proteins; 0/Viral Proteins; 0/gag Gene Products, Human Immunodeficiency Virus; 0/p17 protein, Human Immunodeficiency Virus Type 1 |
| Comments/Corrections | |
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