Document Detail


Functional evidence for indirect recognition of G.U in tRNA(Ala) by alanyl-tRNA synthetase.
MedLine Citation:
PMID:  8539617     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structural features of the G.U wobble pair in Escherichia coli alanine transfer RNA (tRNA(Ala)) that are associated with aminoacylation by alanyl-tRNA synthetase (AlaRS) were investigated in vivo for wild-type tRNA(Ala) and mutant tRNAs with G.U substitutions. tRNA(Ala) with G.U, C.A, or G.A gave similar amounts of charged tRNA(Ala) and supported viability of E. coli lacking chromosomal tRNA(Ala) genes. tRNA(Ala) with G.C was inactive. Recognition of G.U by AlaRS thus requires more than the functional groups on G.U in a regular helix and may involve detection of a helical distortion.
Authors:
K Gabriel; J Schneider; W H McClain
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  271     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-02-08     Completed Date:  1996-02-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  195-7     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, University of Wisconsin, Madison 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Alanine-tRNA Ligase / metabolism*
Anticodon
Base Composition
Base Sequence
Escherichia coli / genetics,  growth & development
Genes, Bacterial
Guanine / chemistry
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Plasmids
RNA, Transfer, Ala / chemistry,  genetics,  metabolism*
Uracil / chemistry
Grant Support
ID/Acronym/Agency:
GM42123/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anticodon; 0/RNA, Transfer, Ala; 66-22-8/Uracil; 73-40-5/Guanine; EC 6.1.1.7/Alanine-tRNA Ligase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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