Document Detail


Functional evidence from microcell-mediated chromosome transfer of myeloid leukemia suppressor genes on human chromosomes 7 and 11.
MedLine Citation:
PMID:  12112528     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The long arm of human chromosome 7 between 7q22 and 7q36 has been identified as a region harboring one or more tumor-suppressor genes (TSGs) inactivated in acute myeloid leukemia (AML). Additional TSGs mapping to other chromosomes may well be involved in the etiology of this disease. For example, experiments using a mouse model system have indicated the possible presence of an AML TSG at 11p11-12. Microcell-mediated chromosome transfer (MMCT) has been used to introduce human chromosomes 7 and 11 into a murine myeloid leukemia cell line. A proportion of MMCT hybrid clones containing either whole chromosome 7 or fragments of chromosome 11 showed a significant delay in leukemogenic onset when injected into syngeneic mice. Screening of hybrid clones did not associate any human microsatellite markers with decreased leukemogenic potential in vivo. However, preliminary evidence was obtained of allelic loss at chromosomal regions homologous with human 7q22 in murine F1 hybrid AMLs. Our data provide functional evidence of AML-associated TSGs localized to human chromosomes 7 and 11 in support of previously published studies on cytogenetic and allelic losses associated with AML development.
Authors:
Jennifer Wilding; Emmy Meijne; Jackie Haines; John Moody; Alan Edwards; Robert F Newbold; Chris Parris; Roger Cox; Andrew Silver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  34     ISSN:  1045-2257     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-11     Completed Date:  2002-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  390-7     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Affiliation:
Radiation Effects Department, National Radiological Protection Board, Chilton, Oxfordshire OX11 0RQ, UK.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
Chimera / genetics
Chromosome Banding
Chromosome Mapping / methods
Chromosomes, Human, Pair 11 / genetics*
Chromosomes, Human, Pair 7 / genetics*
Clone Cells
Cytogenetic Analysis / methods
Flow Cytometry / methods
Gene Transfer Techniques*
Genes, Tumor Suppressor*
Genetic Markers / genetics
Homeodomain Proteins / genetics
Humans
Leukemia, Myeloid / enzymology,  genetics*,  pathology
Loss of Heterozygosity / genetics
Membrane Proteins / genetics
Mice
Mice, Inbred CBA
Neoplasm Transplantation* / methods
Nucleic Acid Amplification Techniques
Peroxidase / metabolism
Polymerase Chain Reaction
Tumor Cells, Cultured
Tumor Suppressor Proteins*
Chemical
Reg. No./Substance:
0/Genetic Markers; 0/Homeodomain Proteins; 0/Membrane Proteins; 0/TES protein, human; 0/Tumor Suppressor Proteins; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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