Document Detail


Functional evidence for a colorectal cancer tumor suppressor gene at chromosome 8p22-23 by monochromosome transfer.
MedLine Citation:
PMID:  8912863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosome 8p is considered, from loss of heterozygosity analysis, to be a strong candidate for the location of a tumor suppressor gene inactivated in colorectal cancer. We have found a 53% (27 of 51) rate of allelic loss at the LPL locus on 8p22, with the smallest region of overlap of deletions including the region D8S258 to D8S277. Using microcell-mediated monochromosome 8 transfer into three colorectal cancer cell lines, SW480, SW620 and HT29, we have demonstrated a reduction of tumorigenicity in SW620 hybrids. Partial deletions of chromosome 8 in some SW620/8 hybrids further delineate the critical region(s) to 8p22-23. Hybrids of the colorectal cancer cell lines SW480 and HT29 containing chromosome 8 did not show suppression of tumorigenesis, but the H29/8 hybrid showed total suppression of soft agar clonicity. This indicates an alternate pathway of mutational progression in these three lines, despite the fact that SW480 was derived from the same patient as SW620.
Authors:
C E Gustafson; P J Wilson; R Lukeis; E Baker; E Woollatt; L Annab; L Hawke; J C Barrett; G Chenevix-Trench
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  56     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-01-07     Completed Date:  1997-01-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5238-45     Citation Subset:  IM    
Affiliation:
The Queensland Institute of Medical Research, The Bancroft Centre, Royal Brisbane Hospital Post Office, Australia.
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MeSH Terms
Descriptor/Qualifier:
Adenoma / genetics
Carcinoma / genetics
Chromosomes, Human, Pair 8 / genetics*
Colorectal Neoplasms / genetics*
Gene Deletion*
Gene Transfer Techniques*
Genes, Tumor Suppressor / genetics*
Genetic Complementation Test
Genetic Markers
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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