Document Detail


Functional effects of nanoparticle exposure on Calu-3 airway epithelial cells.
MedLine Citation:
PMID:  22415089     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High concentrations of manufactured carbon nanoparticles (CNP) are known to cause oxidative stress, inflammatory responses and granuloma formation in respiratory epithelia. To examine the effects of lower, more physiologically relevant concentrations, the human airway epithelial cell line, Calu-3, was used to evaluate potential alterations in transepithelial permeability and cellular function of airway epithelia after exposure to environmentally realistic concentrations of carbon nanoparticles. Three common carbon nanoparticles, fullerenes, single- and multi-wall carbon nanotubes (SWCNT, MWCNT) were used in these experiments. Electrophysiological measurements were performed to assay transepithelial electrical resistance (TEER) and epinephrine-stimulated chloride (Cl(-)) ion secretion of epithelial cell monolayers that had been exposed to nanoparticles for three different times (1 h, 24 h and 48 h) and over a 7 log unit range of concentrations. Fullerenes did not have any effect on the TEER or stimulated ion transport. However, the carbon nanotubes (CNT) significantly decreased TEER and inhibited epinephrine-stimulated Cl(-) secretion. The changes were time dependent and at more chronic exposures caused functional effects which were evident at concentrations substantially lower than have been previously examined. The functional changes manifested in response to physiologically relevant exposures would inhibit mucociliary clearance mechanisms and compromise the barrier function of airway epithelia.
Authors:
Amiraj Banga; Frank A Witzmann; Horia I Petrache; Bonnie L Blazer-Yost
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-01
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  29     ISSN:  1421-9778     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-14     Completed Date:  2012-07-17     Revised Date:  2013-07-31    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  197-212     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
Affiliation:
Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, IN 46202, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Chlorides / metabolism
Epinephrine / pharmacology
Epithelial Cells / drug effects*,  physiology
Fullerenes / chemistry,  toxicity
Humans
Ion Transport / drug effects
Nanoparticles / chemistry,  toxicity*
Nanotubes, Carbon / chemistry,  toxicity
Grant Support
ID/Acronym/Agency:
R01 GM085218/GM/NIGMS NIH HHS; R01GM085218/GM/NIGMS NIH HHS; RC2 ES018810/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Chlorides; 0/Fullerenes; 0/Nanotubes, Carbon; 51-43-4/Epinephrine
Comments/Corrections

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