Document Detail

Functional dissection of the paired segmentation gene in Drosophila embryos.
MedLine Citation:
PMID:  1679407     Owner:  NLM     Status:  MEDLINE    
An ectopic expression assay in Drosophila embryos was used to investigate the roles of pair-rule segmentation genes in the spatial regulation of the segment-polarity gene, engrailed (en). It is hypothesized that the regions of overlap in expression of two genes, paired (prd) and even-skipped (eve), define the odd-numbered en expression stripes. Consistent with this combinatorial model, ectopic expression of prd caused these en stripes to be expanded posteriorly. Surprisingly, however, ectopic expression of a prd gene with a deletion of the conserved paired box resulted in loss of these odd-numbered en stripes. This dominant negative effect is a phenocopy of en expression in prd embryos and suggests that the paired box is necessary for normal prd- function. A similar deletion of odd-numbered en stripes was also observed after ectopic expression of a chimeric fushi tarazu (ftz) gene containing a substituted prd gene homeo box; in addition, in these embryos, the even-numbered en stripes were expanded anteriorly, as observed when the unaltered ftz gene is ectopically expressed. These effects suggest that the chimeric protein may have DNA or protein targets of both the normal Ftz and Prd proteins.
D Morrissey; D Askew; L Raj; M Weir
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genes & development     Volume:  5     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1991-10-09     Completed Date:  1991-10-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1684-96     Citation Subset:  IM    
Department of Biology, Wesleyan University, Middletown, Connecticut 06459.
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MeSH Terms
Cloning, Molecular
Drosophila / embryology,  genetics*
Drosophila Proteins
Fushi Tarazu Transcription Factors
Gene Expression Regulation*
Genes, Homeobox
Heat-Shock Proteins / genetics
Homeodomain Proteins*
Insect Hormones / genetics*
Recombination, Genetic
Transcription Factors / genetics*
Grant Support
Reg. No./Substance:
0/Drosophila Proteins; 0/Fushi Tarazu Transcription Factors; 0/Heat-Shock Proteins; 0/Homeodomain Proteins; 0/Insect Hormones; 0/Transcription Factors; 0/engrail protein, Drosophila; 0/ftz protein, Drosophila

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