Document Detail


Functional dissection of the baculovirus late expression factor-8 gene: sequence requirements for late gene promoter activation.
MedLine Citation:
PMID:  12810876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The late expression factor-8 gene (lef-8) of Autographa californica M nucleopolyhedrovirus encodes the largest subunit of the virally encoded DNA-directed RNA polymerase specific for the transcription of late and very late viral genes. The sequence of lef-8 predicts a C-terminal motif of 13 amino acids that is conserved in other polymerases. Detailed mutagenesis throughout lef-8 was performed, including this C-terminal motif, to define sequences required for late promoter activation. It was found that the conserved C-terminal motif was critical for late gene expression. In addition, regions throughout the entire lef-8-encoding sequence were important for optimal function, suggesting complex protein-protein and protein-DNA interrelationships in the late gene-specific viral transcriptosome.
Authors:
Jane S Titterington; Tamara K Nun; A Lorena Passarelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  84     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-17     Completed Date:  2003-08-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1817-26     Citation Subset:  IM    
Affiliation:
Molecular, Cellular and Developmental Biology Program, Division of Biology, Kansas State University, 232 Ackert Hall, Manhattan, KS 66506-4901, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs*
Animals
Base Sequence
Cell Line
Gene Deletion
Gene Expression Regulation, Viral*
Lepidoptera / virology
Molecular Sequence Data
Mutagenesis, Site-Directed
Nucleopolyhedrovirus / genetics,  metabolism
Promoter Regions, Genetic*
Sequence Analysis, DNA
Transcription, Genetic
Viral Proteins / chemistry,  genetics*,  metabolism*
Grant Support
ID/Acronym/Agency:
1P20RR15563/RR/NCRR NIH HHS; 1P20RR16433/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Viral Proteins; 0/late expression factor 8, Baculovirus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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