Document Detail

Functional coupling of secretion and capacitative calcium entry in PC12 cells.
MedLine Citation:
PMID:  9679028     Owner:  NLM     Status:  MEDLINE    
The caffeine-evoked effects on the intracellular Ca2+ concentration ([Ca2+]i) and on the release of dopamine by PC12 cells were investigated. Stimulation by caffeine resulted in a transient Ca2+ release which was followed by a sustained phase of Ca2+ entry through a non-voltage dependent pathway. Treatment with cyclopiazonic acid (CPA) or thapsigargin, inhibitors of the Ca2+ATPase pump of the endoplasmic reticulum, resulted in only a sustained rise in [Ca2+]i in the presence of extracellular Ca2+. Pretreatment of cells with CPA or thapsigargin abolished the subsequent Ca2+ responses to caffeine. Caffeine also evoked the release of dopamine from the cells only in the presence of extracellular Ca2+, which was mimicked by CPA. These results suggest that store-dependent Ca2+ entry evoked by caffeine has an indispensable role in the secretory response in an excitable cell line, PC12 cells.
S Koizumi; K Inoue
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Publication Detail:
Type:  Corrected and Republished Article; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  247     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-07-20     Completed Date:  1998-07-20     Revised Date:  2007-07-18    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  293-8     Citation Subset:  IM    
Division of Pharmacology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158, Japan.
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MeSH Terms
Caffeine / pharmacology
Calcium / metabolism*
Calcium Channels / drug effects,  metabolism
Calcium-Transporting ATPases / antagonists & inhibitors
Dopamine / metabolism
Extracellular Space / metabolism
Indoles / pharmacology
Inositol 1,4,5-Trisphosphate Receptors
Intracellular Fluid / metabolism
Ion Transport / drug effects
PC12 Cells
Receptors, Cytoplasmic and Nuclear / drug effects,  metabolism
Ryanodine Receptor Calcium Release Channel / drug effects,  metabolism
Signal Transduction
Thapsigargin / pharmacology
Theophylline / pharmacology
Reg. No./Substance:
0/Calcium Channels; 0/Indoles; 0/Inositol 1,4,5-Trisphosphate Receptors; 0/Receptors, Cytoplasmic and Nuclear; 0/Ryanodine Receptor Calcium Release Channel; 18172-33-3/cyclopiazonic acid; 58-08-2/Caffeine; 58-55-9/Theophylline; 67526-95-8/Thapsigargin; 7440-70-2/Calcium; EC ATPases
Corrected and Republished From:
Biochem Biophys Res Commun. 1998 Mar 6;244(1):293-7   [PMID:  9514917 ]

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