Document Detail


Functional coupling of TRPC2 cation channels and the calcium-activated anion channels in rat thyroid cells: Implications for iodide homeostasis.
MedLine Citation:
PMID:  23018590     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The initial step in a synthesis of thyroid hormones is the uptake of iodide from the circulation. Iodide (I(-) ) is transported into thyroid cells via a Na(+) /I(-) symporter (NIS), which is electrogenic and thus sensitive to alterations in membrane potential (V(m) ). I(-) is then released to the lumen of thyroid follicles where the hormones are synthetized and stored. The mechanisms of I(-) release to follicle lumen are poorly characterized. Our whole-cell voltage clamp recordings revealed the presence of a Ca(2+) activated Cl(-) current (CaCC) in Fisher rat thyroid cell line 5 (FRTL-5). Transcripts of anoctamin 1 (ANO1) and anoctamin 10 (ANO10), putative molecular constituents of CaCC, were detected. The anion channels underlying CaCC are highly permeable to I(-) . Both niflumic acid (NFA) and 2-aminoethyl diphenylborinate (2-APB), antagonists of CaCC and transient receptor potential channels, respectively, inhibited CaCC. Canonical transient receptor potential channel 2 (TRPC2) is the only TRPC member present in FRTL-5 cells. The activation rate of CaCC was markedly slower in shTRPC2 knock-down cells, indicating that Ca(2+) entry via TRPC2 contributes to CaCC activation. The uptake of iodide was enhanced and the resting V(m) was more depolarized in TRPC2 knock-down cells. We suggest that the interplay between TRPC2 and ANO1 may have dual effects on iodide transport, modulating I(-) release via ANO channels and I(-) uptake via the V(m) sensitive NIS. J. Cell. Physiol. © 2012 Wiley Periodicals, Inc.
Authors:
Tero Viitanen; Pramod Sukumaran; Christoffer Löf; Kid Törnquist
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Journal of cellular physiology     Volume:  -     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Biosciences, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland; Minerva Foundation Institute for Medical Research, Biomedicum Helsinki, 00270 Helsinki, Finland; Institute of Biomedicine, Physiology, Haartmaninkatu 8 (P.O.BOX 63), 00014 University of Helsinki, Finland.
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