Document Detail

Functional consequences of a neutral pH in neonatal rat stratum corneum.
MedLine Citation:
PMID:  15191554     Owner:  NLM     Status:  MEDLINE    
At birth, neonatal stratum corneum (SC) pH is close to neutral but acidifies with maturation, which can be ascribed, in part, to secretory phospholipase A(2) and sodium/hydrogen antiporter 1 (NHE1) activities. Here we assessed the functional consequences of a neutral SC pH in a newborn rat model. While basal transepidermal water loss rates are near normal, barrier recovery (BR) rates after acute barrier disruption were delayed in newborn animals. The abnormality in barrier homeostasis could be improved by topical applications of an acidic buffer, indicating that barrier abnormality is primarily due to high SC pH. The delay in BR correlated with incompletely processed lamellar membranes and decreased activity of beta-glucocerebrosidase. Inhibition of NHE1 delayed BR after acute barrier perturbation. SC integrity was abnormal in newborn animals. Electron microscopy demonstrated decreased corneodesmosomes (CD) in newborn animals with decreased expression of desmoglein 1 and corneodesmosin. Serine protease activation appears to be responsible for CD degradation in newborn animals, because serine protease activity is increased in the SC and it can be reduced by acidification of the SC. The delay in acidification of neonatal SC results in abnormalities in permeability barrier homeostasis and SC integrity and are likely due to pH-induced modulations in enzyme activity.
Joachim W Fluhr; Man Mao-Qiang; Barbara E Brown; Jean-Pierre Hachem; David G Moskowitz; Marianne Demerjian; Marek Haftek; Guy Serre; Debra Crumrine; Theodora M Mauro; Peter M Elias; Kenneth R Feingold
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  123     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-11     Completed Date:  2004-07-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  140-51     Citation Subset:  IM    
Dermatology and Medical Service, Veterans Affairs Medical Center, San Francisco, California 94121, USA.
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MeSH Terms
Acids / metabolism
Animals, Newborn
Cadherins / metabolism
Desmoglein 1
Desmosomes / metabolism,  ultrastructure
Epidermis / growth & development*,  metabolism*
Glycoproteins / metabolism
Homeostasis / physiology
Hydrogen-Ion Concentration*
Microscopy, Electron
Rats, Sprague-Dawley
Serine Endopeptidases / metabolism
Grant Support
Reg. No./Substance:
0/Acids; 0/CDSN protein, human; 0/Cadherins; 0/Desmoglein 1; 0/Glycoproteins; EC 3.4.21.-/Serine Endopeptidases

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