Document Detail

Functional characterization of a new p53 mutant generated by homozygous deletion in a neuroblastoma cell line.
MedLine Citation:
PMID:  17276397     Owner:  NLM     Status:  MEDLINE    
p53 is a key modulator of a variety of cellular stresses. In human neuroblastomas, p53 is rarely mutated and aberrantly expressed in cytoplasm. In this study, we have identified a novel p53 mutant lacking its COOH-terminal region in neuroblastoma SK-N-AS cells. p53 accumulated in response to cisplatin (CDDP) and thereby promoting apoptosis in neuroblastoma SH-SY5Y cells bearing wild-type p53, whereas SK-N-AS cells did not undergo apoptosis. We found another p53 (p53DeltaC) lacking a part of oligomerization domain and nuclear localization signals in SK-N-AS cells. p53DeltaC was expressed largely in cytoplasm and lost the transactivation function. Furthermore, a 3'-part of the p53 locus was homozygously deleted in SK-N-AS cells. Thus, our present findings suggest that p53 plays an important role in the DNA-damage response in certain neuroblastoma cells and it seems to be important to search for p53 mutations outside DNA-binding domain.
Yohko Nakamura; Toshinori Ozaki; Hidetaka Niizuma; Miki Ohira; Takehiko Kamijo; Akira Nakagawara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-22
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  354     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-13     Completed Date:  2007-04-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  892-8     Citation Subset:  IM    
Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan.
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MeSH Terms
Amino Acid Sequence
Cell Line, Tumor
Cell Survival / drug effects
Cisplatin / pharmacology
DNA Damage
Gene Deletion
Molecular Sequence Data
Neuroblastoma / genetics*
Tumor Suppressor Protein p53 / genetics*
Reg. No./Substance:
0/Tumor Suppressor Protein p53; 15663-27-1/Cisplatin

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