Document Detail

Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide.
MedLine Citation:
PMID:  19308999     Owner:  NLM     Status:  MEDLINE    
Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 microg Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.
Alavala Matta Reddy; Byung Kook Kwak; Hyung Jin Shim; Chiyoung Ahn; Sun Hang Cho; Byung Jin Kim; Sang Young Jeong; Sung-Joo Hwang; Soon Hong Yuk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Contrast media & molecular imaging     Volume:  4     ISSN:  1555-4317     ISO Abbreviation:  Contrast Media Mol Imaging     Publication Date:    2009 May-Jun
Date Detail:
Created Date:  2009-06-15     Completed Date:  2009-09-04     Revised Date:  2010-10-29    
Medline Journal Info:
Nlm Unique ID:  101286760     Medline TA:  Contrast Media Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  118-26     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2009 John Wiley & Sons, Ltd.
Department of Radiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
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MeSH Terms
Biological Markers
Cell Death
Cell Differentiation
Cell Survival
Ferric Compounds / metabolism*
Magnetic Resonance Imaging
Mesenchymal Stem Cells / cytology*,  metabolism*,  ultrastructure
Phantoms, Imaging
Povidone / metabolism*
Staining and Labeling*
Reg. No./Substance:
0/Biological Markers; 0/Ferric Compounds; 1309-37-1/ferric oxide; 9003-39-8/Povidone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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