Document Detail

Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris.
MedLine Citation:
PMID:  16614891     Owner:  NLM     Status:  MEDLINE    
ORF's for rubN6 and rubN4 have been annotated as thymidine diphosphate glucose 4-ketoreductase and thymidine diphosphate glucose 3-aminotransferase by sequence analysis of the rubradirin biosynthetic gene cluster cloned from Streptomyces achromogenes var. rubradiris NRRL 3061. Both ORFs were heterologously expressed in Escherichia coli as His-tagged fusion proteins. The functionalities of TDP-glucose 4-ketoreductase and TDP-glucose 3-aminotransferase were verified by in vitro enzyme assay, and a biosynthetic pathway for TDP-D: -rubranitrose is proposed.
Janardan Lamichhane; Kwangkyoung Liou; Hei Chan Lee; Chun-Gyu Kim; Jae Kyung Sohng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  28     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-14     Completed Date:  2006-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  545-53     Citation Subset:  IM    
Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, IBR, SunMoon University, Tangjeonmyun, 336-708, Asan-si, Chung-nam, Republic of Korea.
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MeSH Terms
Amino Acid Sequence
Bacterial Proteins / genetics,  metabolism
Carbohydrate Dehydrogenases / genetics*,  metabolism
DNA, Bacterial / chemistry,  genetics
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Molecular Structure
Multigene Family / genetics*
Naphthoquinones / chemistry,  metabolism
Oxidoreductases / genetics*,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Streptomyces / enzymology,  genetics*,  metabolism
Transaminases / genetics*,  metabolism
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Naphthoquinones; 11031-38-2/rubradirin; EC 1.-/Oxidoreductases; EC 1.1.-/Carbohydrate Dehydrogenases; EC 1.1.1.-/thymidine diphosphate glucose 4-ketoreductase, Streptomyces achromogenes; EC 2.6.1.-/Transaminases; EC 2.6.1.-/thymidine diphosphate glucose 3-aminotransferase, Streptomyces achromogenes

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