| Functional characterization and hyperosmotic regulation of aquaporin in Synechocystis sp. PCC 6803. | |
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MedLine Citation:
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PMID: 21421383 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The genome of the cyanobacterium Synechocystis sp. PCC 6803 (hereafter, Synechocystis) contains an aqpZ gene (slr2057) which encodes an aquaporin (SsAqpZ), a membrane channel protein that might play a role in osmotic water transport and therefore the growth of Synechocystis. Structural characterization of SsAqpZ by protein sequence analysis and homology modelling revealed that it was more similar to bacterial aquaporin Z than the glycerol facilitator. To understand the functional role of SsAqpZ, the aqpZ knockout (KO) and myc-tagged aqpZ knockin (KI) Synechocystis were constructed. Water channel activity assays showed that SsAqpZ facilitated water transportation. SsAqpZ-mediated changes in cell volume were observed in wild-type (WT) and KI Synechocystis. Expression of SsAqpZ in KI Synechocystis was induced by extracellular hyperosmolarity. In the absence of hyperosmolarity, WT, KO and KI Synechocystis showed the same pattern of growth and no morphological or phenotypical perturbations. Under hyperosmotic condition, while the WT and also KI cells maintained a similar growth rate throughout the entire exponential phase, KO cells grew significantly slower. These results indicate that SsAqpZ has water channel activity and is involved in the adaptation and maintenance of growth of Synechocystis in a hyperosmotic environment. |
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Authors:
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Abul Kalam Azad; Ryosuke Sato; Kai Ohtani; Yoshihiro Sawa; Takahiro Ishikawa; Hitoshi Shibata |
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Publication Detail:
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Type: Journal Article Date: 2010-10-23 |
Journal Detail:
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Title: Plant science (Shannon, Ireland) Volume: 180 ISSN: 0168-9452 ISO Abbreviation: Plant Sci. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-03-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882015 Medline TA: Plant Sci Country: Ireland |
Other Details:
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Languages: eng Pagination: 375-82 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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