| Functional characterization of a ficolin-mediated complement pathway in amphioxus. | |
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MedLine Citation:
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PMID: 21832079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ficolin-mediated complement pathway plays an important role in vertebrate immunity, but it is not clear whether this pathway exists in invertebrates. Here we identified homologs of ficolin pathway components from the cephalochordate amphioxus and investigated whether they had been co-opted into a functional ficolin pathway. Four of these homologs, ficolin FCN1, serine protease MASP1 and MASP3, and complement component C3, were highly expressed in mucosal tissues and gonads, and were significantly up-regulated following bacterial infection. Recombinant FCN1 could induce hemagglutination, discriminate among sugar components, and specifically recognize and aggregate several bacteria (especially gram-positive strains) without showing bactericidal activity. This suggested that FCN1 is a dedicated pattern-recognition receptor. Recombinant serine protease MASP1/3 formed complexes with recombinant FCN1 and facilitated the activation of native C3 protein in amphioxus humoral fluid, in which C3 acted as an immune effector. We conclude that amphioxus have developed a functional ficolin-complement pathway. Because ficolin pathway components have not been reported in non-chordate species, our findings supported the idea that this pathway may represent a chordate-specific innovation in the evolution of the complement system. |
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Authors:
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Huiqing Huang; Shengfeng Huang; Yingcai Yu; Shaochun Yuan; Rui Li; Xin Wang; Hongchen Zhao; Yanhong Yu; Jun Li; Manyi Yang; Liqun Xu; Shangwu Chen; Anlong Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-08-08 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-17 Completed Date: 2011-12-07 Revised Date: 2012-03-12 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 36739-48 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, College of Life Sciences, State Key Laboratory of Biocontrol, National Engineering Research Center of South China Sea Marine Biotechnology, Sun Yat-sen University, Guangzhou 510275, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chordata, Nonvertebrate / genetics, immunology*, metabolism Complement C3 / genetics, immunology*, metabolism Evolution, Molecular* Gene Expression Regulation / physiology* Gram-Positive Bacteria / immunology* Lectins / genetics, immunology*, metabolism Organ Specificity / physiology Recombinant Proteins / genetics, immunology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Complement C3; 0/Lectins; 0/Recombinant Proteins; 0/ficolin |
| Comments/Corrections | |
Erratum In:
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J Biol Chem. 2012 Feb 3;287(6):4394 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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