Document Detail


Functional characterization of the PS II-LHC II supercomplex isolated by a direct method from spinach thylakoid membranes.
MedLine Citation:
PMID:  16228456     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Recently, a novel procedure to isolate a highly pure and active Photosystem II preparation directly from thylakoid membranes, referred to as PS II-LHC II supercomplex, was reported [Eshaghi et al. (1999) FEBS Lett 446: 23-26]. In addition to the reaction center core proteins, the supercomplex contains all the extrinsic proteins of the oxygen evolving complex and a set of chlorophyll a/b binding proteins. In this paper, the functional properties of this isolated supercomplex are further characterized by using EPR spectroscopy, thermoluminescence, fluorescence relaxation kinetics and flash induced oxygen yield measurements. The PS II-LHC II supercomplex contains, in addition to Q(A) and Q(B), a small pool of plastoquinone (PQ). Although the isolated complex is no longer membrane bound, it has preserved functional characteristics of a well defined PS II preparation with the exception of some modification of Q(B) sites.
Authors:
S Eshaghi; E Turcsányi; I Vass; J Nugent; B Andersson; J Barber
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photosynthesis research     Volume:  64     ISSN:  1573-5079     ISO Abbreviation:  Photosyn. Res.     Publication Date:  2000  
Date Detail:
Created Date:  2005-10-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954728     Medline TA:  Photosynth Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  179-87     Citation Subset:  -    
Affiliation:
Wolfson Laboratories, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, SW7 2AY, UK.
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