| Functional characterization of AtHsp90.3 in Saccharomyces cerevisiae and Arabidopsis thaliana under heat stress. | |
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MedLine Citation:
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PMID: 20229063 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The function of cytosolic AtHsp90.3 was characterized by complementing the Saccharomyces cerevisiae endogenous Hsp90 genes and overexpressing it in Arabidopsis thaliana. Though AtHsp90.3 supported the yeast growth under heat stress, in Arabidopsis, compared to the wild type, the transgenic plants overexpressing cytosolic AtHsp90.3 were more sensitive to heat stress with a lower germination rate and higher mortality but and more tolerant to high Ca(2+). Transcriptional expression of heat stress transcription factors, AtHsfA1d, AtHsfA7a and AtHsfB1, and two Hsps, AtHsp101 and AtHsp17, was delayed by constitutive overexpression of cytosolic AtHsp90.3 under heat stress. These results indicate that overexpressing AtHsp90.3 impaired plant tolerance to heat stress and proper homeostasis of Hsp90 was critical for cellular stress response and/or tolerance in plants. |
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Authors:
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Xiangbin Xu; Hongmiao Song; Zhenhua Zhou; Nongnong Shi; Qicai Ying; Huizhong Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-13 |
Journal Detail:
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Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-17 Completed Date: 2010-09-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 979-87 Citation Subset: IM |
Affiliation:
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College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Arabidopsis / genetics, physiology* Arabidopsis Proteins / genetics, metabolism* Calcium / metabolism Cations, Divalent / metabolism Gene Expression Profiling Gene Expression Regulation Genetic Complementation Test HSP90 Heat-Shock Proteins / genetics, metabolism* Hot Temperature* Recombinant Proteins / genetics, metabolism Saccharomyces cerevisiae / genetics, growth & development*, radiation effects* |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Cations, Divalent; 0/HSP90 Heat-Shock Proteins; 0/Hsp90-3 protein, Arabidopsis; 0/Recombinant Proteins; 7440-70-2/Calcium |
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