| Functional characterization of the Aspergillus fumigatus CRZ1 homologue, CrzA. | |
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MedLine Citation:
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PMID: 18298443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The protein phosphatase calcineurin is an important mediator connecting calcium-dependent signalling to various cellular responses in multiple organisms. In fungi calcineurin acts largely through regulating Crz1p-like transcription factors. Here we characterize an Aspergillus fumigatus CRZ1 homologue, CrzA and demonstrate its mediation of cellular tolerance to increased concentrations of calcium and manganese. In addition to acute sensitivity to these ions, and decreased conidiation, the crzA null mutant suffers altered expression of calcium transporter mRNAs under high concentrations of calcium, and loss of virulence when compared with the corresponding complemented and wild-type strains. We use multiple expression analyses to probe the transcriptional basis of A. fumigatus calcium tolerance identifying several genes having calA and/or crzA dependent mRNA accumulation patterns. We also demonstrate that contrary to previous findings, the gene encoding the Aspergillus nidulans calcineurin subunit homologue, cnaA, is not essential and that the cnaA deletion mutant shares the morphological phenotypes observed in the corresponding A. fumigatus mutant, DeltacalA. Exploiting the A. nidulans model system, we have linked calcineurin activity with asexual developmental induction, finding that CrzA supports appropriate developmental induction in a calcineurin and brlA-dependent manner in both species. |
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Authors:
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Frederico Marianetti Soriani; Iran Malavazi; Márcia Eliana da Silva Ferreira; Marcela Savoldi; Marcia Regina Von Zeska Kress; Maria Helena de Souza Goldman; Omar Loss; Elaine Bignell; Gustavo Henrique Goldman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-20 |
Journal Detail:
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Title: Molecular microbiology Volume: 67 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-28 Completed Date: 2008-07-31 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1274-91 Citation Subset: IM |
Affiliation:
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Faculdade de Ciências Farmacêuticas de Ribeirão PretoUniversidade de São Paulo, São Paulo, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus fumigatus
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drug effects,
genetics*,
metabolism Aspergillus nidulans / drug effects, genetics, metabolism Calcineurin / genetics*, metabolism Calcium Chloride / pharmacology Fungal Proteins / genetics*, metabolism, physiology Gene Expression Regulation, Fungal / drug effects Manganese / pharmacology Microscopy, Confocal Mutation RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/RNA, Messenger; 10043-52-4/Calcium Chloride; 7439-96-5/Manganese; EC 3.1.3.16/Calcineurin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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