Document Detail


Functional characteristics of photochemically treated platelets.
MedLine Citation:
PMID:  14996186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A photochemical treatment (PCT) process using the psoralen compound amotosalen HCL (S59) and long wavelength UVA light was developed for inactivation of infectious pathogens and WBCs. In this study the effect of PCT on functional characteristics of the platelets was evaluated in vitro. STUDY DESIGN AND METHODS: Platelet concentrates were treated photochemically using the experimental clinical processing system T-bag S59 Reduction Device (SRD) (n = 4) or the commercially available integral processing system Wafer SRD (n = 4) and compared with control platelet concentrates in plasma/PAS III alone (n = 4). The evaluation included variables with respect to the overall quality of the product (e.g., HSR, pH), the function (aggregation and activation tests), apoptosis (annexin V and caspase 3), and lysis. RESULTS: No differences were found in the product quality variables, in P-selectin expression, and the apoptosis variables. PCT using the T-bag SRD led to a significant decrease in aggregation capacity with collagen and thrombin and a significant increase in plasma LDH, whereas no differences for the Wafer SRD were found. CONCLUSION: PCT using the experimental T-bag SRD led to a significant decrease in platelet function. However, the commercially available Wafer SRD had only minor in vitro effects on the quality of the platelets.
Authors:
Gerard A J Jansen; Huub H D M van Vliet; Hans Vermeij; Erik A M Beckers; Frank W G Leebeek; Pieter Sonneveld; Dick J van Rhenen
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  44     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-03     Completed Date:  2004-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  313-9     Citation Subset:  IM    
Affiliation:
Sanquin Blood Bank South West Region, Wytemaweg 10, 3015 CN Rotterdam, the Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Bacterial Infections / prevention & control*
Blood Platelets / drug effects*,  physiology
Blood Preservation
Humans
Photochemistry
Platelet Aggregation / drug effects
Psoralens*
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Psoralens; 0/S-59, psoralen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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