| Functional assessment of skeletal muscle in intact mice lacking myostatin by concurrent NMR imaging and spectroscopy. | |
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MedLine Citation:
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PMID: 20010628 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inhibiting myostatin (mstn) causes spectacular increase in muscle mass, spurring research for therapeutic approaches against neuromuscular disorders. Yet, possible functional deterioration and compromised force production have been reported in isolated muscle of null mstn(-/-) mice. We analyzed vascular and metabolic response to repeated electro-stimulated exercise in vivo in mstn(-/-) mice compared with FVB wild-type controls (WT), using interleaved multi-parametric functional nuclear magnetic resonance (NMR) imaging and spectroscopy. At steady-state exercise, specific force of plantar flexion, phosphocreatine consumption measured by phosphorus spectroscopy and maximum perfusion measured by arterial spin-labeled (ASL) NMR imaging were identical in both groups. After exercise, phosphorus spectroscopy revealed reduced oxidative mitochondrial capacity in mstn(-/-), whereas early recovery perfusion was identical and oxygen extraction, estimated from the blood oxygen level-dependent (BOLD) contrast, was decreased when compared with WT. Hyperemia was prolonged, indicating specific regulation of the perfusional response in mstn(-/-) mice. Histology showed an increased proportion of type IIb fibers in hypertrophied muscles, but the distribution of capillary contacts per fiber between oxidative and glycolytic fibers was unaltered in mstn(-/-) compared with WT. These integrated results formed coherent evidence of a congruous, non-pathologic shift toward a more glycolytic metabolism in this model of mstn(-/-). |
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Authors:
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C Baligand; H Gilson; J C M?nard; O Schakman; C Wary; J-P Thissen; P G Carlier |
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Publication Detail:
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Type: Journal Article Date: 2009-12-10 |
Journal Detail:
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Title: Gene therapy Volume: 17 ISSN: 1476-5462 ISO Abbreviation: Gene Ther. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-11 Completed Date: 2010-06-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421525 Medline TA: Gene Ther Country: England |
Other Details:
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Languages: eng Pagination: 328-37 Citation Subset: IM |
Affiliation:
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Institute of Myology, NMR Laboratory, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Exercise Test Glycolysis / genetics Hyperemia / metabolism Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Male Mice Mice, Knockout Muscle, Skeletal / blood supply, metabolism, physiology* Myostatin / deficiency*, genetics Phosphocreatine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Myostatin; 67-07-2/Phosphocreatine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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