Document Detail


Functional aspects of megamitochondria isolated from hydrazine- and ethanol-treated rat livers.
MedLine Citation:
PMID:  10692174     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It is essential to analyze functions of megamitochondria (MG) to elucidate the mechanism of the formation of MG induced under various pathological conditions. The MG fraction obtained by a routine isolation procedure for normal mitochondria always consists of a mixed population of mitochondria enlarged to various degrees and also normal-sized ones. The purpose of the present study is to answer the question of whether or not data obtained from the MG fraction consisting of such a heterogeneous population of mitochondria with respect to their sizes really reflect functions of MG. In the present study mitochondria were obtained from the livers of rats treated with a 1% hydrazine diet for 8 days and those given 32% ethanol in drinking water for up to 2 months using various isolation procedures. Results obtained are summarized as follows: (i) mitochondria enlarged to various degrees and normal-sized ones are sometimes connected with each other by a narrow stalk in the hepatocyte of hydrazine-treated animals, and such connections are maintained to some extent when mitochondria are isolated; and (ii) mitochondria obtained from experimental animals by a routine isolation procedure for mitochondria ((700-7000)gR2"') and those obtained by alternative isolation procedure yielding the heavy ((500-2000)gR2"') and light ((2000-7000)gR2"') fractions show some functional similarities: decreases in the content of cytochrome a + a3; decreases in oxygen consumptions and phosphorylating abilities; decreases in monoamine oxidase and cytochrome c oxidase activities; lowered membrane potential of mitochondria; decreases in the rate of the generation of reactive oxygen species. These results may suggest that mitochondria enlarged to various degrees and normal-sized ones are functionally similar to each other and that the MG fraction obtained by a routine isolation procedure for normal mitochondria can be applied to the study of the function of MG.
Authors:
T Wakabayashi; M A Teranishi; M Karbowski; Y Nishizawa; J Usukura; C Kurono; T Soji
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pathology international     Volume:  50     ISSN:  1320-5463     ISO Abbreviation:  Pathol. Int.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-05-09     Completed Date:  2000-05-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9431380     Medline TA:  Pathol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  20-33     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Molecular Pathology, Nagoya University School of Medicine, Nagoya, Japan. twakaba@tsuru.med.nagoya-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation
Electron Transport Complex IV / metabolism
Ethanol / pharmacology*
Flow Cytometry
Hydrazines / pharmacology*
Liver / cytology,  drug effects*
Male
Membrane Potentials / drug effects
Mitochondria, Liver / physiology*,  ultrastructure
Monoamine Oxidase / metabolism
Oxidative Phosphorylation
Oxygen Consumption / drug effects
Rats
Rats, Wistar
Reactive Oxygen Species
Chemical
Reg. No./Substance:
0/Hydrazines; 0/Reactive Oxygen Species; 302-01-2/hydrazine; 64-17-5/Ethanol; EC 1.4.3.4/Monoamine Oxidase; EC 1.9.3.1/Electron Transport Complex IV

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