Document Detail


Functional analysis to identify genes in wine yeast adaptation to low temperature fermentation.
MedLine Citation:
PMID:  22507142     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims: To identify genes and proteins involved in adaptation to low temperature fermentations in a commercial wine yeast Methods and Results: Nine proteins were identified as representing the most significant changes in proteomic maps during the first 24 hours of fermentation at low (13 °C) and standard temperature (25 °C). These proteins were mainly involved in stress response and glucose and nitrogen metabolism. Transcription analysis of the genes encoding most of these proteins within the same time frame of wine fermentation presented a good correlation with proteomic data. Knockout and over-expressing strains of some of these genes were constructed and tested to evaluate their ability to start the fermentation process. The strain over-expressing ILV5 improved its fermentation activity in the first hours of fermentation. This strain showed a quicker process of mitochondrial degeneration, an altered intracellular amino acid profile and laxer nitrogen catabolite repression regulation. Conclusions: The use of proteomic and transcriptomic analysis is useful to detect key molecular adaptation mechanisms of biotechnological interest for industrial processes. ILV5 gene seems to be important in wine yeast adaptation to low temperature fermentation. Significance and Impact of the Study: This study provides information that might help improve the future performance of wine yeast, either by genetic modification or by adaptation during industrial production. © 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Authors:
Z Salvadó; R Chiva; N Rozès; R Cordero-Otero; J M Guillamón
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-16
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.
Affiliation:
Biotecnologia Enològica. Departament de Bioquímica i Biotecnologia, Facultat de Enologia, Universitat Rovira i Virgili. Marcel·li Domingo s/n, 43007, Tarragona, Spain. Departamento de Biotecnología de los alimentos, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avda. Agustín Escardino, 7, E-46980, Paterna, Valencia, Spain.
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