| Functional analysis of the orf390 gene of the white spot syndrome virus. | |
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MedLine Citation:
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PMID: 20362018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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orf390 (WSSV449) is a novel apoptosis inhibitor gene in the genome of the White Spot Syndrome Virus (WSSV). In the present study, we focus on the function of orf390 gene. Stable expression of orf390 prevented SF9 insect cells from undergoing actinomycin D-induced apoptosis. ORF390 also rescued the replication of a p35-deficient-mutant (AcMNPVDeltap35k/pol+) in SF9 cells. In addition, ORF390 inhibits the activities of caspase-3 and -9 in vivo and in vitro. Here we demonstrate that the anti-apoptotic activity of ORF390 is dependent on two putative caspase-9 cleavage sites (VETD233 downward arrowG and LEHD303 downward arrowG) and one caspase-3 cleavage site (DEVD272 downward arrowG). Our results support the conclusion that these three sites play a key role in the suppression of apoptosis mediated by ORF390. These data further suggest that orf390 encodes a novel anti-apoptotic protein involved in cell survival and apoptosis regulation. |
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Authors:
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Feng Yan; Ding Xia; Songya Lv; Yipeng Qi; Hua Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-31 |
Journal Detail:
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Title: Virus research Volume: 151 ISSN: 1872-7492 ISO Abbreviation: Virus Res. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-14 Completed Date: 2010-08-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 39-44 Citation Subset: IM |
Affiliation:
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Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caspase 1 / antagonists & inhibitors Caspase 3 / antagonists & inhibitors Caspase 9 / antagonists & inhibitors Cell Line Genes, Viral* Humans Viral Proteins / genetics, metabolism* White spot syndrome virus 1 / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/ORF390 protein, shrimp white spot syndrome virus; 0/Viral Proteins; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 9; EC 3.4.22.36/Caspase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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