Document Detail

Functional analysis of the murine T-cell receptor beta enhancer and characteristics of its DNA-binding proteins.
MedLine Citation:
PMID:  2144608     Owner:  NLM     Status:  MEDLINE    
The minimal T-cell receptor (TCR) beta-chain (TCR beta) enhancer has been identified by transfection into lymphoid cells. The minimal enhancer was active in T cells and in some B-lineage cells. When a larger fragment containing the minimal enhancer was used, its activity was apparent only in T cells. Studies with phytohemagglutinin and 4 beta-phorbol-12,13-dibutyrate revealed that the enhancer activity was increased by these agents. By a combination of DNase I footprinting, gel mobility shift assay, and methylation interference analysis, seven different motifs were identified within the minimal enhancer. Furthermore, competition experiments showed that some of these elements bound identical or similar factors that are known to bind to the TCR V beta promoter decamer or to the immunoglobulin enhancer kappa E2 or muEBP-E motif. These shared motifs may be important in the differential gene activity among the different lymphoid subsets.
J Takeda; A Cheng; F Mauxion; C A Nelson; R D Newberry; W C Sha; R Sen; D Y Loh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  10     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-10-18     Completed Date:  1990-10-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5027-35     Citation Subset:  IM    
Howard Hughes Medical Institute, Washington University School of Medicine, St. Louis, Missouri 63110.
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MeSH Terms
Base Sequence
Binding Sites
Cloning, Molecular
DNA Mutational Analysis
DNA-Binding Proteins / physiology*
Enhancer Elements, Genetic*
Gene Expression Regulation
Lymphocytes / physiology*
Molecular Sequence Data
Phorbol 12,13-Dibutyrate / pharmacology
Phytohemagglutinins / pharmacology
Promoter Regions, Genetic
Receptors, Antigen, T-Cell / genetics*
Receptors, Antigen, T-Cell, alpha-beta
Restriction Mapping
Structure-Activity Relationship
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Phytohemagglutinins; 0/Receptors, Antigen, T-Cell; 0/Receptors, Antigen, T-Cell, alpha-beta; 37558-16-0/Phorbol 12,13-Dibutyrate

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