Document Detail


Functional analysis of lipid metabolism in Magnaporthe grisea reveals a requirement for peroxisomal fatty acid beta-oxidation during appressorium-mediated plant infection.
MedLine Citation:
PMID:  17506326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rice blast fungus Magnaporthe grisea infects plants by means of specialized infection structures known as appressoria. Turgor generated in the appressorium provides the invasive force that allows the fungus to breach the leaf cuticle with a narrow-penetration hypha gaining entry to the underlying epidermal cell. Appressorium maturation in M. grisea involves mass transfer of lipid bodies to the developing appressorium, coupled to autophagic cell death in the conidium and rapid lipolysis at the onset of appressorial turgor generation. Here, we report identification of the principal components of lipid metabolism in M. grisea based on genome sequence analysis. We show that deletion of any of the eight putative intracellular triacylglycerol lipase-encoding genes from the fungus is insufficient to prevent plant infection, highlighting the complexity and redundancy associated with appressorial lipolysis. In contrast, we demonstrate that a peroxisomally located multifunctional, fatty acid beta-oxidation enzyme is critical to appressorium physiology, and blocking peroxisomal biogenesis prevents plant infection. Taken together, our results indicate that, although triacylglycerol breakdown in the appressorium involves the concerted action of several lipases, fatty acid metabolism and consequent generation of acetyl CoA are necessary for M. grisea to complete its prepenetration phase of development and enter the host plant.
Authors:
Zheng-Yi Wang; Darren M Soanes; Michael J Kershaw; Nicholas J Talbot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular plant-microbe interactions : MPMI     Volume:  20     ISSN:  0894-0282     ISO Abbreviation:  Mol. Plant Microbe Interact.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-17     Completed Date:  2007-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9107902     Medline TA:  Mol Plant Microbe Interact     Country:  United States    
Other Details:
Languages:  eng     Pagination:  475-91     Citation Subset:  IM    
Affiliation:
School of Biosciences, University of Exeter, Washington Singer Laboratories, Perry Road, Exeter, EX4 4QG, UK.
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MeSH Terms
Descriptor/Qualifier:
Cell Wall / metabolism,  ultrastructure
Fatty Acids / metabolism*
Fungal Proteins / genetics,  metabolism
Gene Expression Regulation, Fungal
Genome, Fungal
Green Fluorescent Proteins / genetics,  metabolism
Lipase / genetics,  metabolism
Lipid Metabolism / physiology*
Magnaporthe / genetics,  growth & development,  metabolism*
Microscopy, Electron
Microscopy, Fluorescence
Mutation
Onions / microbiology
Oryza sativa / microbiology
Oxidation-Reduction
Peroxisomes / enzymology,  metabolism*
Plant Diseases / microbiology
Plants / microbiology*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fungal Proteins; 147336-22-9/Green Fluorescent Proteins; EC 3.1.1.3/Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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