| Functional analysis of the endoxylanase B (xynB) promoter from Penicillium purpurogenum. | |
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MedLine Citation:
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PMID: 18661134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In Penicillium purpurogenum, the gene encoding endoxylanase B (xynB) is highly expressed by xylan and repressed by glucose at the transcriptional level. The promoter of this gene has a modular structure, with eight putative XlnR binding sites in tandem (XlnR module), and upstream from them, four putative CreA binding sites (CreA module). Promoter fragments containing different modules were inserted into a plasmid, pAN49-1, which contains a basal fungal promoter linked to a reporter gene (lacZ) and its expression was studied in vivo in Aspergillus nidulans. The XlnR module is able to trigger high beta-galactosidase activity in the presence of xylan, but the lack of most XlnR sites notoriously reduces this enzymatic activity. No enzyme induction is observed if the orientation of the promoter fragment is inverted. The presence of the CreA module is necessary for glucose repression when beta-galactosidase activity is previously induced by xylan. However, when transformant strains containing the XlnR module but lacking all CreA sites were grown in glucose without pre-induction in xylan, a low beta-galactosidase activity was observed compared with the same transformants grown in xylan. These results agree with a double-lock regulatory mechanism for both direct and indirect repression of xylanolytic genes by glucose. |
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Authors:
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Jheimmy Díaz; Renato Chávez; Luis F Larrondo; Jaime Eyzaguirre; Paulina Bull |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-26 |
Journal Detail:
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Title: Current genetics Volume: 54 ISSN: 0172-8083 ISO Abbreviation: Curr. Genet. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-22 Completed Date: 2008-11-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8004904 Medline TA: Curr Genet Country: United States |
Other Details:
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Languages: eng Pagination: 133-41 Citation Subset: IM |
Affiliation:
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Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Alameda 340, Casilla 114-D, Santiago, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus
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genetics,
growth & development Base Sequence Blotting, Southern DNA Primers Endo-1,4-beta Xylanases / genetics* Genes, Fungal Genes, Reporter Glucuronidase / genetics Penicillium / genetics* Plasmids Promoter Regions, Genetic* Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; EC 3.2.1.31/Glucuronidase; EC 3.2.1.8/Endo-1,4-beta Xylanases |
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