Document Detail


Functional analysis of Arabidopsis mutants points to novel roles for glutathione in coupling H(2)O(2) to activation of salicylic acid accumulation and signaling.
MedLine Citation:
PMID:  23148658     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Through its interaction with H(2)O(2), glutathione is a candidate for transmission of signals in plant responses to pathogens, but identification of signaling roles is complicated by its antioxidant function. Using a genetic approach based on a conditional catalase-deficient Arabidopsis mutant, cat2, this study aimed at establishing whether GSH plays an important functional role in the transmission of signals downstream of H(2)O(2).
RESULTS: Introducing the cad2 or allelic mutations in the glutathione synthesis pathway into cat2 blocked H(2)O(2)-triggered GSH oxidation and accumulation. While no effects on NADP(H) or ascorbate were observed, and H(2)O(2)-induced decreases in growth were maintained, blocking GSH modulation antagonized salicylic acid (SA) accumulation and SA-dependent responses. Other novel double and triple mutants were produced and compared with cat2 cad2 at the levels of phenotype, expression of marker genes, nontargeted metabolite profiling, accumulation of SA, and bacterial resistance. Most of the effects of the cad2 mutation on H(2)O(2)-triggered responses were distinct from those produced by mutations for GLUTATHIONE REDUCTASE1 (GR1) or NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1), and were linked to compromised induction of ISOCHORISMATE SYNTHASE1 (ICS1) and ICS1-dependent SA accumulation.
INNOVATION: A novel genetic approach was used in which GSH content or antioxidative capacity was independently modified in an H(2)O(2) signaling background. Analysis of new double and triple mutants allowed us to infer previously undescribed regulatory roles for GSH.
CONCLUSION: In parallel to its antioxidant role, GSH acts independently of NPR1 to allow increased intracellular H(2)O(2) to activate SA signaling, a key defense response in plants.
Authors:
Yi Han; Sejir Chaouch; Amna Mhamdi; Guillaume Queval; Bernd Zechmann; Graham Noctor
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-14
Journal Detail:
Title:  Antioxidants & redox signaling     Volume:  18     ISSN:  1557-7716     ISO Abbreviation:  Antioxid. Redox Signal.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-18     Completed Date:  2013-10-17     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  100888899     Medline TA:  Antioxid Redox Signal     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2106-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  metabolism*
Glutathione / metabolism*
Hydrogen Peroxide / metabolism*
Mutation*
Oxidative Stress
Salicylic Acid / metabolism*
Signal Transduction*
Grant Support
ID/Acronym/Agency:
P 22988-B16//Austrian Science Fund FWF
Chemical
Reg. No./Substance:
BBX060AN9V/Hydrogen Peroxide; GAN16C9B8O/Glutathione; O414PZ4LPZ/Salicylic Acid
Comments/Corrections

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