| Functional activation of glutamate ionotropic receptors in the developing mouse retina. | |
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MedLine Citation:
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PMID: 17177257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ionotropic glutamate receptors have been associated with early development of the visual process by regulating cell differentiation, cell motility, and synaptic contacts. We determined the expression of functional ionotropic glutamate receptors during development of the mouse retina by assessing 1-amino-4-guanidobutane (agmatine; AGB) immunolabelling after application of a range of glutamate analogs. Colocalization of AGB with calretinin and islet-1 allowed the identification of functional receptors in neurochemically defined neurons. Activation with kainate (KA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), and N-methyl-D-aspartate (NMDA) resulted in AGB entry into neurons consistent with that found previous receptor subunit localization studies in the developing retina. Temporal analysis revealed that application of 50 microM KA activated receptors as early as embryonic day 18 in the ventricular zone and in the ganglion cell layer, whereas 30 muM AMPA activated cells predominantly in the ganglion cell layer. Cholinergic amacrine cells showed functional KA and AMPA receptors upon their insertion into the conventional amacrine cell layer from postnatal day 1 (P1). OFF cone bipolar cells showed functional KA receptors from P6, at a developmental age when they are known to make contact with ganglion cells. NMDA activation led to diffuse AGB labeling at birth among cells in the ganglion cell layer, whereas, at P1, regularly spaced cholinergic amacrine cells in the conventional amacrine cell layer started to be responsive to NMDA. Non-NMDA receptors were first to show functional activation in the developing retina, and cholinergic amacrine cells displayed functional ionotropic glutamate receptors after reaching their final destination. |
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Authors:
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Monica L Acosta; Jacqueline Chua; Michael Kalloniatis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 500 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2006-12-26 Completed Date: 2007-02-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: United States |
Other Details:
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Languages: eng Pagination: 923-41 Citation Subset: IM |
Copyright Information:
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2006 Wiley-Liss, Inc. |
Affiliation:
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Department of Optometry and Vision Science, University of Auckland, Auckland, New Zealand. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium-Binding Protein, Vitamin D-Dependent / metabolism Homeodomain Proteins / metabolism Immunohistochemistry Mice Mice, Inbred C57BL Neurons / metabolism* Receptors, AMPA / metabolism* Receptors, Kainic Acid / metabolism* Receptors, N-Methyl-D-Aspartate / metabolism* Retina / cytology, growth & development, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Homeodomain Proteins; 0/Receptors, AMPA; 0/Receptors, Kainic Acid; 0/Receptors, N-Methyl-D-Aspartate; 0/calretinin; 0/insulin gene enhancer binding protein Isl-1 |
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