Document Detail

Functional screening of intracardiac cell transplants using two-photon fluorescence microscopy.
MedLine Citation:
PMID:  22481568     Owner:  NLM     Status:  MEDLINE    
Although the adult mammalian myocardium exhibits a limited ability to undergo regenerative growth, its intrinsic renewal rate is insufficient to compensate for myocyte loss during cardiac disease. Transplantation of donor cardiomyocytes or cardiomyogenic stem cells is considered a promising strategy for reconstitution of cardiac mass, provided the engrafted cells functionally integrate with host myocardium and actively contribute to its contractile force. The authors previously developed a two-photon fluorescence microscopy-based assay that allows in situ screening of donor cell function after intracardiac delivery of the cells. This report reviews the techniques of two-photon fluorescence microscopy and summarizes its application for quantifying the extent to which a variety of donor cell types stably and functionally couple with the recipient myocardium.
Wen Tao; Mark H Soonpaa; Loren J Field; Peng-Sheng Chen; Anthony B Firulli; Weinian Shou; Michael Rubart
Related Documents :
1234108 - The distribution of serotonin-containing cells in the human alimentary tract.
21566928 - Synergism of cytotoxicity between cis-diaminedichloro-platinum-(ii) and cis-diamine(1,1...
21965738 - Modulation of multidrug efflux pump activity by new hydantoin derivatives on colon aden...
9773938 - Unusual gastric polyp showing submucosal proliferation of glands: case report and liter...
18044978 - Sulfur x-ray absorption spectroscopy of living mammalian cells: an enabling tool for su...
2807488 - Tumour induction in fetal brain transplants exposed to the viral oncogenes polyoma midd...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-04-06
Journal Detail:
Title:  Pediatric cardiology     Volume:  33     ISSN:  1432-1971     ISO Abbreviation:  Pediatr Cardiol     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-10     Completed Date:  2013-04-25     Revised Date:  2013-10-22    
Medline Journal Info:
Nlm Unique ID:  8003849     Medline TA:  Pediatr Cardiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  929-37     Citation Subset:  IM    
Department of Pediatrics, Riley Heart Research Center, Indiana University School of Medicine, 1044 West Walnut Street, Indianapolis, IN 46202, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Calcium / metabolism*
Microscopy, Fluorescence / methods*
Myocardium / cytology*,  metabolism
Myocytes, Cardiac / metabolism,  transplantation*
Grant Support
P01 HL085098-040001/HL/NHLBI NIH HHS; P01 HL085098-05/HL/NHLBI NIH HHS; R01 AR061392/AR/NIAMS NIH HHS; R01 HL081092/HL/NHLBI NIH HHS; R01 HL083126/HL/NHLBI NIH HHS; R01HL075165/HL/NHLBI NIH HHS
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Can spin-polarized photoemission measure spin properties in condensed matter?
Next Document:  Biological applications of magnetic nanoparticles.