Document Detail


Functional regulatory divergence of the innate immune system in interspecific Drosophila hybrids.
MedLine Citation:
PMID:  20551040     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to investigate divergence of immune regulation among Drosophila species, we have engaged in a study of innate immune function in F1 hybrids of Drosophila melanogaster and D. simulans. If pathways have diverged between the species such that incompatibilities have arisen between interacting components of the immune network, we expect the hybrids to display dysregulation of immune genes. We have quantified gene induction in hybrid and parental flies in response to bacterial infection. These results show that although the hybrids do not suffer widespread immune breakdown, they show significantly different regulation of many immune genes relative to the parents. We examine this divergence in terms of additivity and expression differences among genes, observing distinct patterns of dysregulation among functional groups within the pathways of the innate immune system. The functional groups most sensitive to misexpression in the hybrids are the downstream components of the network, indicative of some propagation of dysregulation throughout the immune pathways. Interestingly, this dysregulation does not appear to associate with phenotypic differences in bacterial load after infection in hybrids, possibly highlighting some robustness of function of the innate immune response to perturbations like hybridization.
Authors:
Erin M Hill-Burns; Andrew G Clark
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-14
Journal Detail:
Title:  Molecular biology and evolution     Volume:  27     ISSN:  1537-1719     ISO Abbreviation:  Mol. Biol. Evol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2011-01-27     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8501455     Medline TA:  Mol Biol Evol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2596-605     Citation Subset:  IM    
Affiliation:
Field of Genetics and Development, Department of Molecular Biology and Genetics, Cornell University. erinhill@life.bio.sunysb.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Infections / genetics,  immunology
Bacterial Load / genetics,  immunology
Crosses, Genetic
Drosophila / classification,  genetics*,  immunology*,  microbiology
Drosophila melanogaster / genetics,  immunology,  microbiology
Female
Gene Expression Regulation
Genes, Insect / genetics
Genetic Variation*
Hybridization, Genetic*
Immunity, Innate / genetics*
Male
Signal Transduction / genetics
Species Specificity
Grant Support
ID/Acronym/Agency:
R01 AI064890/AI/NIAID NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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