Document Detail

Functional HSF1 requires aromatic-participant interactions in protecting mouse embryonic fibroblasts against apoptosis via G2 cell cycle arrest.
MedLine Citation:
PMID:  22526392     Owner:  NLM     Status:  MEDLINE    
The present study highlighted the aromatic-participant interactions in in vivo trimerization of HSF1 and got an insight into the process of HSF1 protecting against apoptosis. In mouse embryonic fibroblasts (MEFs), mutations of mouse HSF1 (W37A, Y60A and F104A) resulted in a loss of trimerization activity, impaired binding of the heat shock element (HSE) and lack of heat shock protein 70 (HSP70) expression after a heat shock. Under UV irradiation, wild-type mouse HSF1 protected the MEFs from UV-induced apoptosis, but none of the mutants offered protection. We found that normal expression of HSF1 was essential to the cell arrest in G2 phase, assisting with the cell cycle checkpoint. The cells that lack normal HSF1 failed to arrest in the G2 phase, resulting in the process of cell apoptosis. We conclude that the treatment with UV or heat shock stresses appears to induce the approach of HSF1 monomers directly via aromatic-participant interactions, followed by the formation of a HSF1 trimer. HSF1 protects the MEFs from the stresses through the expression of HSPs and a G2 cell cycle arrest.
Ziwei Chang; Ming Lu; Sung-Min Park; Hyun-Kyung Park; Ho Sung Kang; Youngshang Pak; Jang-Su Park
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-04-17
Journal Detail:
Title:  Molecules and cells     Volume:  33     ISSN:  0219-1032     ISO Abbreviation:  Mol. Cells     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-17     Completed Date:  2012-12-07     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  465-70     Citation Subset:  IM    
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MeSH Terms
Amino Acids / metabolism
Apoptosis / genetics,  physiology*
Cell Cycle Checkpoints / genetics
Cell Death / genetics
Cells, Cultured
DNA-Binding Proteins / genetics*,  metabolism*
Fibroblasts / cytology,  metabolism,  physiology*
G2 Phase Cell Cycle Checkpoints / genetics*
HSP70 Heat-Shock Proteins / genetics,  metabolism
Heat-Shock Response / genetics
Protein Binding
Protein Multimerization
Transcription Factors / genetics*,  metabolism*
Reg. No./Substance:
0/Amino Acids; 0/DNA-Binding Proteins; 0/HSP70 Heat-Shock Proteins; 0/Hsf1 protein, mouse; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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