Document Detail


Functional evaluation of neural stem cell differentiation by single cell calcium imaging.
MedLine Citation:
PMID:  21476976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neurogenesis in the adult mammalian brain occurs in two specific brain areas, the subventricular zone (SVZ) bordering the lateral ventricles and the subgranular zone (SGZ) of the hippocampus. Although these regions are prone to produce new neurons, cultured cells from these neurogenic niches tend to be mixed cultures, containing both neurons and glial cells. Several reports highlight the potential of the self-healing capacity of the brain following injury. Even though much knowledge has been produced on the neurogenesis itself, brain repairing strategies are still far away from patients cure. Here we review general concepts in the neurogenesis field, also addressing the methods available to study neural stem cell differentiation. A major problem faced by research groups and companies dedicated to brain regenerative medicine resides on the lack of good methods to functionally identify neural stem cell differentiation and novel drug targets. To address this issue, we developed a unique single cell calcium imaging-based method to functionally discriminate different cell types derived from SVZ neural stem cell cultures. The unique functional profile of each SVZ cell type was correlated at the single cell level with the immunodetection of specific phenotypic markers. This platform was raised on the basis of the functional response of neurons, oligodendrocytes and immature cells to depolarising agents, to thrombin and to histamine, respectively. We also outline key studies in which our new platform was extremely relevant in the context of drug discovery and development in the area of brain regenerative medicine.
Authors:
Maria Francisca Eiriz; Sofia Grade; Alexandra Rosa; Sara Xapelli; Liliana Bernardino; Fabienne Agasse; João O Malva
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Current stem cell research & therapy     Volume:  6     ISSN:  1574-888X     ISO Abbreviation:  Curr Stem Cell Res Ther     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-10     Completed Date:  2012-01-09     Revised Date:  2012-01-19    
Medline Journal Info:
Nlm Unique ID:  101272517     Medline TA:  Curr Stem Cell Res Ther     Country:  United Arab Emirates    
Other Details:
Languages:  eng     Pagination:  288-96     Citation Subset:  IM    
Affiliation:
Neuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, University of Coimbra, Largo Marquês de Pombal, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / physiology
Calcium Signaling*
Cell Differentiation*
Hippocampus / cytology,  physiology
Humans
Lateral Ventricles / cytology,  physiology
Neural Stem Cells / cytology*,  physiology
Neurogenesis
Regeneration
Single-Cell Analysis / methods*

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