Document Detail

Functional domain analysis of the Remorin protein LjSYMREM1 in Lotus japonicus.
MedLine Citation:
PMID:  22292047     Owner:  NLM     Status:  MEDLINE    
In legumes rhizobial infection during root nodule symbiosis (RNS) is controlled by a conserved set of receptor proteins and downstream components. MtSYMREM1, a protein of the Remorin family in Medicago truncatula, was shown to interact with at least three receptor-like kinases (RLKs) that are essential for RNS. Remorins are comprised of a conserved C-terminal domain and a variable N-terminal region that defines the six different Remorin groups. While both N- and C-terminal regions of Remorins belonging to the same phylogenetic group are similar to each other throughout the plant kingdom, the N-terminal domains of legume-specific group 2 Remorins show exceptional high degrees of sequence divergence suggesting evolutionary specialization of this protein within this clade. We therefore identified and characterized the MtSYMREM1 ortholog from Lotus japonicus (LjSYMREM1), a model legume that forms determinate root nodules. Here, we resolved its spatio-temporal regulation and showed that over-expression of LjSYMREM1 increases nodulation on transgenic roots. Using a structure-function approach we show that protein interactions including Remorin oligomerization are mainly mediated and stabilized by the Remorin C-terminal region with its coiled-coil domain while the RLK kinase domains transiently interact in vivo and phosphorylate a residue in the N-terminal region of the LjSYMREM1 protein in vitro. These data provide novel insights into the mechanism of this putative molecular scaffold protein and underline its importance during rhizobial infection.
Katalin Tóth; Thomas F Stratil; Esben B Madsen; Juanying Ye; Claudia Popp; Meritxell Antolín-Llovera; Christina Grossmann; Ole N Jensen; Arthur Schüssler; Martin Parniske; Thomas Ott
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-23
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-31     Completed Date:  2012-06-04     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e30817     Citation Subset:  IM    
Department of Genetics, University of Munich, Martinsried, Germany.
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MeSH Terms
Carrier Proteins / chemistry*,  genetics,  metabolism,  physiology*
Evolution, Molecular
Fabaceae / genetics,  metabolism
Gene Expression Regulation, Plant
Genetic Speciation
Lotus* / genetics,  metabolism,  physiology
Medicago truncatula / genetics,  metabolism
Phosphoproteins / chemistry*,  genetics,  metabolism,  physiology*
Plant Proteins / chemistry*,  genetics,  metabolism,  physiology*
Plants, Genetically Modified
Protein Binding
Protein Multimerization / genetics
Protein Structure, Tertiary / physiology
Root Nodules, Plant / genetics,  metabolism
Structure-Activity Relationship
Symbiosis / genetics,  physiology
Reg. No./Substance:
0/Carrier Proteins; 0/Phosphoproteins; 0/Plant Proteins; 0/remorin

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