Document Detail


Functional domain analysis of the Remorin protein LjSYMREM1 in Lotus japonicus.
MedLine Citation:
PMID:  22292047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In legumes rhizobial infection during root nodule symbiosis (RNS) is controlled by a conserved set of receptor proteins and downstream components. MtSYMREM1, a protein of the Remorin family in Medicago truncatula, was shown to interact with at least three receptor-like kinases (RLKs) that are essential for RNS. Remorins are comprised of a conserved C-terminal domain and a variable N-terminal region that defines the six different Remorin groups. While both N- and C-terminal regions of Remorins belonging to the same phylogenetic group are similar to each other throughout the plant kingdom, the N-terminal domains of legume-specific group 2 Remorins show exceptional high degrees of sequence divergence suggesting evolutionary specialization of this protein within this clade. We therefore identified and characterized the MtSYMREM1 ortholog from Lotus japonicus (LjSYMREM1), a model legume that forms determinate root nodules. Here, we resolved its spatio-temporal regulation and showed that over-expression of LjSYMREM1 increases nodulation on transgenic roots. Using a structure-function approach we show that protein interactions including Remorin oligomerization are mainly mediated and stabilized by the Remorin C-terminal region with its coiled-coil domain while the RLK kinase domains transiently interact in vivo and phosphorylate a residue in the N-terminal region of the LjSYMREM1 protein in vitro. These data provide novel insights into the mechanism of this putative molecular scaffold protein and underline its importance during rhizobial infection.
Authors:
Katalin Tóth; Thomas F Stratil; Esben B Madsen; Juanying Ye; Claudia Popp; Meritxell Antolín-Llovera; Christina Grossmann; Ole N Jensen; Arthur Schüssler; Martin Parniske; Thomas Ott
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-23
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-31     Completed Date:  2012-06-04     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e30817     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Munich, Martinsried, Germany.
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MeSH Terms
Descriptor/Qualifier:
Carrier Proteins / chemistry*,  genetics,  metabolism,  physiology*
Evolution, Molecular
Fabaceae / genetics,  metabolism
Gene Expression Regulation, Plant
Genetic Speciation
Lotus* / genetics,  metabolism,  physiology
Medicago truncatula / genetics,  metabolism
Phosphoproteins / chemistry*,  genetics,  metabolism,  physiology*
Phylogeny
Plant Proteins / chemistry*,  genetics,  metabolism,  physiology*
Plants, Genetically Modified
Protein Binding
Protein Multimerization / genetics
Protein Structure, Tertiary / physiology
Root Nodules, Plant / genetics,  metabolism
Structure-Activity Relationship
Symbiosis / genetics,  physiology
Transfection
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Phosphoproteins; 0/Plant Proteins; 0/remorin
Comments/Corrections

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