Document Detail

Functional Characterization of Rice Genes Using a Gene-Indexed T-DNA Insertional Mutant Population.
MedLine Citation:
PMID:  23135844     Owner:  NLM     Status:  In-Data-Review    
Despite the availability of the finished genome sequence and tools for its analyses, few rice genes have been characterized. Because Agrobacterium-mediated transformation causes random T-DNA insertions across the genome, T-DNA can be a good mutagen for functional genomics. Gene-indexed mutants with flanking sequences around inserted T-DNA are valuable resources for accelerating functional characterizations of rice genes. Such mutant lines, covering at least half the rice genome, have been generated through international efforts. Here, we describe approaches that use functional genomics with T-DNA insertional mutagenesis.
Ki-Hong Jung; Gynheung An
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  956     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-67     Citation Subset:  IM    
Department of Plant Pathology, University of California, Davis, CA, USA.
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