| Functional capacity of human islets after long-distance shipment and encapsulation. | |
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MedLine Citation:
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PMID: 20966806 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: : Human islets produced at an isolation center are shipped to researchers, usually taking short periods to arrive at their destination. In this study, we investigated whether human islets after long-distance shipment across the Pacific Ocean for 2 to 3 days and encapsulation could maintain their functionality. METHODS: : Human islets were encapsulated in alginate and viability assessed using carboxyfluorescein diacetate and propidium iodide. Stimulation index after static glucose incubation was calculated. Streptozotocin-induced diabetic nonobese diabetic/severe combined immunodeficient mice were transplanted with 3000, 2000, or 1000 islet equivalents of nonencapsulated and encapsulated islets and glucose levels monitored. When levels were normal, the graft was retrieved and assessed. RESULTS: : Viability of human islets was unaltered after long-distance shipment with a retrieval rate of 88.3% ± 1.9%. After encapsulation, the viability was unchanged (before encapsulation 86.1% ± 0.7% vs after encapsulation 80.8 ± 0.7%) at 11 days after isolation. Function in vitro of nonencapsulated and encapsulated islets was unaffected with a stimulation index of 2.2 and 1.9, respectively. Euglycemia was achieved in 100% mice receiving 2000 and 3000 islet equivalents of nonencapsulated and encapsulated islets, respectively. Capsules retrieved after transplantation was intact, free floating, and contained viable islets. CONCLUSION: : Human islets can be shipped safely for long distances without compromising viability and function even after encapsulation and culture. |
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Authors:
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Vijayaganapathy Vaithilingam; Barbara Barbaro; Jose Oberholzer; Bernard E Tuch |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Pancreas Volume: 40 ISSN: 1536-4828 ISO Abbreviation: Pancreas Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: United States |
Other Details:
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Languages: eng Pagination: 247-52 Citation Subset: IM |
Affiliation:
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From the *Department of Pharmacology, University of New South Wales; †Australian Foundation for Diabetes Research, Sydney, Australia; and ‡Department of Surgery, University of Illinois at Chicago, Chicago, IL. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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