| Functional CCR5 receptor protects patients with arthritis from high synovial burden of infecting Chlamydia trachomatis. | |
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MedLine Citation:
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PMID: 20811274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: The CCR5 chemokine receptor occurs in a wild-type (wt) and a nonfunctional deleted form (Δ32). Reports suggested that Chlamydia-induced reproductive tract pathology is attenuated in women bearing Δ32. The authors asked whether the mutation affects synovial prevalence and burden of Chlamydia trachomatis. METHODS: Polymerase chain reaction (PCR) defined CCR5 genotype in synovial tissue DNA from 218 individuals: 21 controls, 110 with reactive arthritis (ReA), 83 with undifferentiated oligoarthritis (UO), 4 with osteoarthritis (OA). Disease durations were 0.5 to 21 years. Additional PCR assays defined the presence of C trachomatis DNA. Bacterial load was assessed by real-time PCR in selected samples. RESULTS: Five controls were wt/Δ32, 16 were wt/wt; 2 of 21 controls (both wt/wt) were PCR positive for C trachomatis. Eighty-five (44%) patients with arthritis were PCR positive for C trachomatis (69 ReA and 16 UO). For patients with ReA, 14 (13%) had wt/Δ32, 10 (71%) of whom were PCR positive. Nineteen patients with UO (23%) were wt/Δ32, with 1 (1%) PCR positive. No differences existed for gender or other factors. One patient with OA had wt/Δ32. In ReA and UO samples, wt/Δ32 heterozygotes had a 5- to 10-fold higher bacterial burden than did wt/wt patients (P = 0.03), regardless of diagnosis. CONCLUSION: These results indicate that the wt/wt genotype is associated with attenuated synovial bacterial load compared with loads in wt/Δ32 patients. Although no alleles other than Δ32 were assessed, our data suggest that this allele provides little/no protection from ReA in patients infected with Chlamydia- but it may provide some protection in patients with UO. The basis of this possible differential effect of CCR5 genotype is under study. |
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Authors:
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Hervé C Gérard; Jessica A Stanich; Cynthia E Oszust; Judith A Whittum-Hudson; John D Carter; H Ralph Schumacher; Alan P Hudson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: The American journal of the medical sciences Volume: 340 ISSN: 1538-2990 ISO Abbreviation: Am. J. Med. Sci. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-01 Completed Date: 2011-01-06 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 0370506 Medline TA: Am J Med Sci Country: United States |
Other Details:
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Languages: eng Pagination: 448-51 Citation Subset: AIM; IM |
Affiliation:
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Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Arthritis
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immunology* Arthritis, Reactive / prevention & control* Chlamydia Infections / immunology* Chlamydia trachomatis* Female Genotype Humans Male Receptors, CCR5 / genetics, physiology* Synovial Membrane / microbiology* |
| Grant Support | |
ID/Acronym/Agency:
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AR-42541/AR/NIAMS NIH HHS; AR-47186/AR/NIAMS NIH HHS; AR-48331/AR/NIAMS NIH HHS; AR-53646/AR/NIAMS NIH HHS; R01 AR042541-14/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, CCR5 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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