Document Detail


Higher-level production of volatile fatty acids in vitro by chicken gut microbiotas than by human gut microbiotas as determined by functional analyses.
MedLine Citation:
PMID:  22685152     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to determine the relationship between the composition and function of gut microbiota. Here, we compared the bacterial compositions and fermentation metabolites of human and chicken gut microbiotas. Results generated by quantitative PCR (qPCR) and 454 pyrosequencing of the 16S rRNA gene V3 region showed the compositions of human and chicken microbiotas to be markedly different, with chicken cecal microbiotas displaying more diversity than human fecal microbiotas. The nutrient requirements of each microbiota growing under batch and chemostat conditions were analyzed. The results showed that chicken cecal microbiotas required simple sugars and peptides to maintain balanced growth in vitro but that human fecal microbiotas preferred polysaccharides and proteins. Chicken microbiotas also produced higher concentrations of volatile fatty acids than did human microbiotas. Our data suggest that the availability of different fermentable substrates in the chicken cecum, which exist due to the unique anatomical structure of the cecum, may provide an environment favorable to the nourishment of microbiotas suited to the production of the higher-energy metabolites required by the bird. Therefore, gut structure, nutrition, immunity, and life-style all contribute to the selection of an exclusive bacterial community that produces types of metabolites beneficial to the host.
Authors:
Fang Lei; Yeshi Yin; Yuezhu Wang; Bo Deng; Hongwei David Yu; Lanjuan Li; Charlie Xiang; Shengyue Wang; Baoli Zhu; Xin Wang
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-08
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-11-28     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5763-72     Citation Subset:  IM    
Affiliation:
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / isolation & purification,  metabolism*
Biodiversity*
Carbohydrate Metabolism
Chickens
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Fatty Acids, Volatile / metabolism*
Feces / microbiology
Fermentation
Gastrointestinal Tract / microbiology*
Humans
Metagenome
Proteins / metabolism
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Fatty Acids, Volatile; 0/Proteins; 0/RNA, Ribosomal, 16S
Comments/Corrections

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