Document Detail

Function and significance of very low density lipoprotein receptor subtype II.
MedLine Citation:
PMID:  16201256     Owner:  NLM     Status:  MEDLINE    
To explore the functions of very low density lipoprotein receptor (VLDL-R) subtype II in lipoprotein metabolism and foam cells formation, the recombinant plasmid with the two subtypes cDNA was constructed respectively, the 1dl-A7 cell lines were transfected and two cell lines expressing VLDL-R were obtained: one stably expressing the VLDLR with the O-linked sugar region (type I VLDLR) and the other without the O-linked sugar region (type II VLDLR). In the study on binding of VLDLR to their nuclein labeled natural ligands (VLDL and beta-VLDL), it was found that surface binding of 25 I-VLDL or 125 I-beta-VLDL of 1dl-A7 cells transfected with type I VLDL R recombinant (1dl-A7-VRI) was more higher than that of 1dl-A7 cells transfected with type II VLDLR recombinant (1dl-A7-VRII). After being incubated with VLDL for different time, the contents of triglyceride and total cholesterol in cells were mensurated, and the formation of foam cells and accumulation of lipid in cells was observed by oil-red O staining. The results showed that the contents of triglyceride and total cholesterol in 1dl-A7-VR I were much higher than those in 1dl-A7-VR II, and 1dl-A7-VR I could transform into foam cells notably. It was suggested that type I VLDLR binds with relative higher affinity to VLDL and beta-VLDL, and internalizes much more lipoprotein into cells. As a result, we can conclude that type I VLDLR plays a more important role in lipoprotein metabolism and foam cells formation than type II VLDLR.
Jun Tian; Hao Bi; Yinghong Li; Pu Yang; Yiqiang Zong; Yu Wang; Shen Qu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  25     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2005  
Date Detail:
Created Date:  2005-10-05     Completed Date:  2007-10-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  229-33     Citation Subset:  IM    
Department of Biochemistry and Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
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MeSH Terms
Arteriosclerosis / metabolism,  pathology
CHO Cells
Cells, Cultured
Cloning, Molecular
Foam Cells / cytology*,  metabolism
Lipoproteins, VLDL / metabolism*
Receptors, LDL / classification,  metabolism,  physiology*
Triglycerides / metabolism
Reg. No./Substance:
0/Ligands; 0/Lipoproteins, VLDL; 0/Receptors, LDL; 0/Triglycerides; 0/VLDL receptor

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