Document Detail

Function of a eukaryotic transcription activator during the transcription cycle.
MedLine Citation:
PMID:  15866178     Owner:  NLM     Status:  MEDLINE    
Site-specific photocrosslinkers positioned within the central transcription-activating region of yeast Gcn4 were used to identify, in an unbiased way, three polypeptides in direct physical proximity to the activator during the process of transcription activation. Crosslinking was specific and did not change during different steps of the transcription cycle. The crosslinking targets were identified as Tra1, Gal11, and Taf12, subunits of four complexes (SAGA, NuA4, Mediator, and TFIID) known to play a role in gene regulation. Using this crosslinking assay, an activating region mutant, and extracts depleted of individual complexes containing the crosslinking targets, we found that contact with Tra1/SAGA is critical for activation, Gal11 contact has a modest effect on activation, and contact with TFIID and NuA4 is of little or no importance for activation under our conditions. Thus, a single activating region contacts multiple factors, and each contact makes differential contributions to transcriptional activation.
James Fishburn; Neeman Mohibullah; Steven Hahn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular cell     Volume:  18     ISSN:  1097-2765     ISO Abbreviation:  Mol. Cell     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-06-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-78     Citation Subset:  IM    
Howard Hughes Medical Institute, 1100 Fairview Ave North, Mailstop A1-162, Seattle, Washington 98109, USA.
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MeSH Terms
Amino Acid Sequence
Cross-Linking Reagents / metabolism
DNA-Binding Proteins / chemistry*,  metabolism
Gene Expression Regulation*
Molecular Sequence Data
Multiprotein Complexes
Peptides / metabolism*
Protein Kinases / chemistry*,  metabolism
Protein Subunits / metabolism
Saccharomyces cerevisiae Proteins / chemistry*,  metabolism
Transcription Factor TFIID / genetics,  metabolism
Transcription, Genetic*
Grant Support
R01 GM053451-08/GM/NIGMS NIH HHS; R01 GM053451-09/GM/NIGMS NIH HHS; R01 GM053451-10/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Cross-Linking Reagents; 0/DNA-Binding Proteins; 0/Multiprotein Complexes; 0/Peptides; 0/Protein Subunits; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factor TFIID; EC 2.7.-/Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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