| Comparison of the function of the serotonin transporter in the vasculature of male and female rats. | |
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MedLine Citation:
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PMID: 21371073 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. The serotonin transporter (SERT) handles serotonin (5-hydroxytryptamine (5-HT)) and is blocked by the antidepressant SERT inhibitors fluoxetine and fluvoxamine. Although the importance of SERT in the central nervous system is clear, SERT also functions in the peripheral vasculature. In the present study, we tested the hypothesis that the vasculature from female rats has increased SERT function compared with male rats because females are more responsive to SERT inhibitors. 2. In addition to in vitro experiments, in vivo experiments were used to evaluate how male and female rats handle chronically elevated levels of 5-HT. Wild-type (WT) and SERT-knockout (SERT-KO) rats were infused with 5-HT (25 μg/kg per min) for 7 days by minipump. 3. Using HPLC analysis, we demonstrated that blood vessels (aorta, carotid artery, jugular vein and vena cava) from naïve, non-infused female rats took up 5-HT acutely in vitro in a SERT-dependent manner. In in vitro experiments, SERT affected the contractility of aortas from female rats, as evidenced by an eightfold increase in potency of 5-HT in fluvoxamine (1 μmol/L)-incubated WT aortas compared with control. Fluvoxamine did not alter 5-HT-induced contraction in aortas from SERT-KO female rats. 4. Infusion of 5-HT resulted in an increase in tissue 5-HT that was reduced to a larger extent in blood vessels from female than male SERT-KO rats. Aortic contractions to 5-HT were abolished in aortas from male and female 5-HT-infused SERT-KO rats compared with WT rats. 5. Collectively, these data suggest that SERT function, when challenged with 5-HT, is modestly more important in the vasculature of the female compared with male rat. |
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Authors:
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Aurea Elizabeth Linder; Robert Patrick Davis; Robert Burnett; Stephanie W Watts |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental pharmacology & physiology Volume: 38 ISSN: 1440-1681 ISO Abbreviation: Clin. Exp. Pharmacol. Physiol. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-05-03 Completed Date: 2011-09-06 Revised Date: 2012-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0425076 Medline TA: Clin Exp Pharmacol Physiol Country: Australia |
Other Details:
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Languages: eng Pagination: 314-22 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Clinical and Experimental Pharmacology and Physiology © 2011 Blackwell Publishing Asia Pty Ltd. |
Affiliation:
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Department of Pharmacology, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil. wattss@msu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / physiology Blood Pressure / genetics, physiology Blood Vessels / metabolism*, physiology Female Heart Rate / genetics, physiology Isometric Contraction / genetics Male Motor Activity / genetics, physiology Rats Rats, Sprague-Dawley Rats, Transgenic Rats, Wistar Serotonin / blood, metabolism Serotonin Plasma Membrane Transport Proteins / genetics, metabolism, physiology* Sex Characteristics* |
| Grant Support | |
ID/Acronym/Agency:
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F30 HL099024/HL/NHLBI NIH HHS; HL081115/HL/NHLBI NIH HHS; R01 HL081115-04/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Serotonin Plasma Membrane Transport Proteins; 50-67-9/Serotonin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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