| Fumonisin B1-induced apoptosis in neuroblastoma, glioblastoma and hypothalamic cell lines. | |
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MedLine Citation:
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PMID: 16860453 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fumonisin B(1) (FB(1)) is a mycotoxin produced by Fusarium verticilliodes, which commonly infects corn across the world. Fusarium fungi may also be found in moisture-damaged buildings. In this study, we investigated the role of apoptosis in the toxicity of FB(1) in four different cell lines. Activation of caspase-3-like protease, DNA fragmentation and expression of p53 and Bcl-2 family proteins were studied in mouse GT1-7 hypothalamic, rat C6 glioblastoma, human U-118MG glioblastoma, and human SH-SY5Y neuroblastoma cells exposed to 0.1-100microM FB(1) for 0-144h. Caspase-3-like protease activity increased in all cell lines, except SH-SY5Y, at 48-144h, and internucleosomal DNA fragmentation occurred in all of the cell lines, pointing to a role for apoptosis in the toxicity of FB(1). However, the expressions of p53 or pro- or antiapoptotic Bcl-2 family proteins (Bax, Bcl-2, Bcl-X(L) and Mcl-1) were not affected in any of the cell lines even after prolonged exposure to FB(1) at high doses. The results of this study, together with the results of our previous studies, provide evidence that FB(1) is a potential neurotoxin, but that the toxicity of FB(1) varies between different cell lines. The sensitivity of these cell lines towards FB(1) is as follows: U-118MG>GT1-7>C6>SH-SY5Y cells. These results are consistent with the assumption that cells of glial origin may be more sensitive towards FB(1) than cells of neural origin. |
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Authors:
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Helene Stockmann-Juvala; Jonne Naarala; Jarkko Loikkanen; Kirsi Vähäkangas; Kai Savolainen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-06-13 |
Journal Detail:
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Title: Toxicology Volume: 225 ISSN: 0300-483X ISO Abbreviation: Toxicology Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-07-31 Completed Date: 2006-09-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0361055 Medline TA: Toxicology Country: Ireland |
Other Details:
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Languages: eng Pagination: 234-41 Citation Subset: IM |
Affiliation:
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Unit of Excellence for Immunotoxicology, Finnish Institute of Occupational Health, Topeliuksenkatu 41 a A, 00250 Helsinki, Finland. helene.stockmann-juvala@ttl.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspase 3 Caspases / biosynthesis Cell Line, Tumor / drug effects, metabolism, pathology Cell Survival / drug effects DNA Fragmentation Dose-Response Relationship, Drug Fumonisins / toxicity* Glioblastoma / drug therapy*, pathology Humans Hypothalamus / drug effects*, pathology Mycotoxins / toxicity* Neuroblastoma / drug therapy*, pathology Proto-Oncogene Proteins c-bcl-2 / metabolism Rats Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fumonisins; 0/Mycotoxins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 116355-83-0/fumonisin B1; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Casp3 protein, mouse; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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